HORMONAL REGULATION OF INNER EAR FLUID IN THE INTERMEDIATE CELLS OF THE STRIA VASCULARIS

临床上，我们已报道内淋巴积水(如梅尼埃病)患者血浆抗利尿激素(ADH)升高。在本项目中，给豚鼠注射ADH，以揭示ADH是否与内淋巴积液的产生有关。ADH致豚鼠内淋巴积水。然后，用ADH拮抗剂阻断内淋巴囊和内淋巴管，使豚鼠出现内淋巴下垂。ADH拮抗剂使Reissner膜折叠，减少内淋巴积水。这些结果表明，内淋巴的产生可能受激素即ADH的调节。我们推测，这种ADH的作用部位可能是血管纹中的中间细胞，因为中间细胞包围着血管纹中的毛细血管。目前，我们还不能分离出这个中间细胞。我们正在修改中间细胞分离技术…更多的QUE。我们将继续研究ADH对分离的中间细胞的影响。结合其他相关研究，我们研究了“人工内淋巴注入耳蜗管对耳蜗内电位的影响”和“细长的周细胞样细胞连接沙土鼠和大鼠耳蜗纹中离散的毛细血管”。在人工内淋巴注入耳蜗管对耳蜗内电位的影响中，将人工内淋巴注入内淋巴间隙。注射人工内淋巴可引起缺氧时内淋巴电位(EP)波幅的一过性升高和负EP波幅的降低。在“沙土鼠和大鼠耳蜗纹中细长的周细胞样细胞连接离散的毛细血管”中，对沙土鼠和大鼠的耳蜗纹中离散的毛细血管之间的桥接结构进行了形态学研究。透射电子显微镜切片显示，基膜周围的细长细胞为桥接结构提供了结构基础。免疫荧光法显示基底板，毛细血管常在桥接细胞的附着处弯曲。较少

Clinically, we have reported that plasma antidiuretic hormone (ADH) increased in patients with endolymphatic hydrops (e. g. Meniere's disease). In this project, guinea pigs were administrated ADH in order to reveal if ADH has any relations to produce endolymphatic hydrops. Administration of ADH produced endolymphatic hydrops in guinea pigs. Next, an antagonist of ADH was administered in guinea pig with endolymphatic hvdrops, which was made by obstruction of the endolymphatic sac andlor duct. The antagonist of ADH made folding of the Reissner membrane, which means the endolymphatic hydrops, was reduced. These results suggest that production of the endolymph may be regulated by hormone i.e. ADH.We suspect the effecting site of this ADH may he the intermediate cell in the stria vascularis because the intermediate cells surround the capillary in the stria vascularis. For the time being, we could not make isolate this intermediate cell. We are revising the intermediate cell isolation techni … More que. We will continue this project which is an investigation of effects of ADH on the isolated intermediate cells.Concerning to other related investigations, we researched "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential" and "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats". In "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential", artificial endolymph was injected into the endolymphatic space. The injection of artificial endolymph produced transient increase of endolymphatic potential (EP) amplitude and decrease of negative EP amplitude during anoxia. In "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats", bridging structures between discrete capillaries in the stria vascularis were studied morphologically in gerbils and rats. Strial thin sections for transmission electron microscopy revealed that elongated cells surrounded by the basal lamina provided the structural basis for the bridging structure. Visualization of the basal lamina by immunofluorescent methods revealed that capillaries were often bent at the site of attachment of the bridging cell. Less