HORMONAL REGULATION OF INNER EAR FLUID IN THE INTERMEDIATE CELLS OF THE STRIA VASCULARIS

临床上，我们已经报道了血浆抗利尿激素（ADH）增加，在患者的内淋巴积水（e。G.梅尼埃病）。本课题通过给豚鼠注射抗利尿激素，探讨抗利尿激素是否与内淋巴积水的发生有关。给药ADH可使豚鼠出现内淋巴积水。然后，用内淋巴滴给豚鼠注射抗利尿激素拮抗剂，通过阻塞内淋巴囊和/或内淋巴管制备内淋巴滴。ADH拮抗剂使Reissner膜折叠，即内淋巴积水减轻。这些结果表明，内淋巴的产生可能受激素ADH的调节，由于血管纹内的中间细胞围绕着毛细血管，推测ADH的作用部位可能是血管纹内的中间细胞。目前，我们还不能分离出这种中间细胞。我们正在修订中间细胞分离技术， 关于我们 que的本课题将继续进行ADH对耳蜗中间细胞的影响研究，并结合其他相关研究，对“人工内淋巴注入耳蜗导管对耳蜗内电位的影响”和“延长的周细胞样细胞连接沙土鼠和大鼠耳蜗血管纹中离散的毛细血管”进行了研究。在“人工内淋巴注入耳蜗导管对耳蜗内电位的影响”中，将人工内淋巴注入内淋巴间隙。注入人工内淋巴可使缺氧时内淋巴电位（EP）幅值一过性增高，负EP幅值降低。在“Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats”中，对沙鼠和大鼠耳蜗血管纹中离散毛细血管之间的桥接结构进行了形态学研究。透射电子显微镜下的肌层薄切片显示，由基膜包围的细长细胞为桥接结构提供了结构基础。通过免疫荧光方法观察基底层，发现毛细血管在桥细胞附着的部位经常弯曲。少

Clinically, we have reported that plasma antidiuretic hormone (ADH) increased in patients with endolymphatic hydrops (e. g. Meniere's disease). In this project, guinea pigs were administrated ADH in order to reveal if ADH has any relations to produce endolymphatic hydrops. Administration of ADH produced endolymphatic hydrops in guinea pigs. Next, an antagonist of ADH was administered in guinea pig with endolymphatic hvdrops, which was made by obstruction of the endolymphatic sac andlor duct. The antagonist of ADH made folding of the Reissner membrane, which means the endolymphatic hydrops, was reduced. These results suggest that production of the endolymph may be regulated by hormone i.e. ADH.We suspect the effecting site of this ADH may he the intermediate cell in the stria vascularis because the intermediate cells surround the capillary in the stria vascularis. For the time being, we could not make isolate this intermediate cell. We are revising the intermediate cell isolation techni … More que. We will continue this project which is an investigation of effects of ADH on the isolated intermediate cells.Concerning to other related investigations, we researched "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential" and "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats". In "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential", artificial endolymph was injected into the endolymphatic space. The injection of artificial endolymph produced transient increase of endolymphatic potential (EP) amplitude and decrease of negative EP amplitude during anoxia. In "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats", bridging structures between discrete capillaries in the stria vascularis were studied morphologically in gerbils and rats. Strial thin sections for transmission electron microscopy revealed that elongated cells surrounded by the basal lamina provided the structural basis for the bridging structure. Visualization of the basal lamina by immunofluorescent methods revealed that capillaries were often bent at the site of attachment of the bridging cell. Less