HORMONAL REGULATION OF INNER EAR FLUID IN THE INTERMEDIATE CELLS OF THE STRIA VASCULARIS

血管纹中间细胞内耳液的激素调节

• 批准号: 09671750
• 负责人: KAKIGI Akinobu
• 金额: $ 1.98万
• 依托单位: KOCHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671750/
• 关键词: ENDOLYMPHATIC HYDROPS; ANTIDIURETIC HORMONE; STRIA VASCULARIS; INTERMEDIATE CELL; ENDOCOCHLEAR POTENTIAL; ARTIFICIAL ENDOLYMP; 辺緑細胞

Clinically, we have reported that plasma antidiuretic hormone (ADH) increased in patients with endolymphatic hydrops (e. g. Meniere's disease). In this project, guinea pigs were administrated ADH in order to reveal if ADH has any relations to produce endolymphatic hydrops. Administration of ADH produced endolymphatic hydrops in guinea pigs. Next, an antagonist of ADH was administered in guinea pig with endolymphatic hvdrops, which was made by obstruction of the endolymphatic sac andlor duct. The antagonist of ADH made folding of the Reissner membrane, which means the endolymphatic hydrops, was reduced. These results suggest that production of the endolymph may be regulated by hormone i.e. ADH.We suspect the effecting site of this ADH may he the intermediate cell in the stria vascularis because the intermediate cells surround the capillary in the stria vascularis. For the time being, we could not make isolate this intermediate cell. We are revising the intermediate cell isolation techni … More que. We will continue this project which is an investigation of effects of ADH on the isolated intermediate cells.Concerning to other related investigations, we researched "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential" and "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats". In "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential", artificial endolymph was injected into the endolymphatic space. The injection of artificial endolymph produced transient increase of endolymphatic potential (EP) amplitude and decrease of negative EP amplitude during anoxia. In "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats", bridging structures between discrete capillaries in the stria vascularis were studied morphologically in gerbils and rats. Strial thin sections for transmission electron microscopy revealed that elongated cells surrounded by the basal lamina provided the structural basis for the bridging structure. Visualization of the basal lamina by immunofluorescent methods revealed that capillaries were often bent at the site of attachment of the bridging cell. Less

临床上，我们报道了内淋巴水肿（如梅尼埃病）患者血浆抗利尿激素（ADH）升高。在本项目中，我们给豚鼠注射了ADH，以揭示ADH是否与内淋巴水肿的产生有任何关系。豚鼠注射ADH可产生内淋巴积液。接下来，通过内淋巴囊和内淋巴管阻塞，给豚鼠内淋巴滴注ADH拮抗剂。ADH拮抗剂使赖斯纳膜折叠，这意味着内淋巴水肿减少。这些结果表明，内淋巴的产生可能受到激素即ADH的调节。我们怀疑这种ADH的作用部位可能是血管纹的中间细胞，因为中间细胞包围着血管纹的毛细血管。目前，我们还不能分离出这种中间细胞。我们正在改进中间细胞分离技术…我们将继续研究ADH对分离的中间细胞的影响。在其他相关研究中，我们研究了“人工内淋巴注入耳蜗管对耳蜗电位的影响”和“沙鼠和大鼠耳蜗血管纹中细长周细胞样细胞连接离散毛细血管”。在“人工内淋巴注入耳蜗管对耳蜗电位的影响”一文中，将人工内淋巴注入内淋巴间隙。注射人工内淋巴后，缺氧时内淋巴电位（EP）幅值短暂升高，负EP幅值降低。在“沙鼠和大鼠耳蜗血管纹中细长的周细胞样细胞连接离散毛细血管”一文中，对沙鼠和大鼠耳蜗血管纹中离散毛细血管之间的桥接结构进行了形态学研究。透射电镜试片显示，被基板包围的细长细胞为桥接结构提供了结构基础。免疫荧光法显示，桥接细胞附着部位的毛细血管经常弯曲。少

项目成果

Motonori Ando, Akinobu Kakigi, Shunji Takeuchi: "Elongated pericyte-like cells connect disdrete capillaries in the cochlear stria vascularis of gerbils and rats" Cell & Tissue Research. (in press).

Motonori Ando、Akinobu Kakigi、Shunji Takeuchi：“细长的周细胞样细胞连接沙鼠和大鼠耳蜗血管纹中的离散毛细血管” Cell

Akinobu Kakigi: "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential" Hearing Research. 116. 34-38 (1998)

Akinobu Kakigi：“人工内淋巴注射到耳蜗管对耳蜗内电位的影响”听力研究。

Motonori Ando: "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats" Cell & Tissue Research. 掲載予定. (1999)

Motonori Ando：“细长的周细胞样细胞连接沙鼠和大鼠耳蜗血管纹中的离散毛细血管”，细胞与组织研究（1999 年）。

Motonori Ando: "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats" Cell & Tissue Research. (掲載予定). (1999)

Motonori Ando：“细长的周细胞样细胞连接沙鼠和大鼠耳蜗血管纹中的离散毛细血管”，《细胞与组织研究》（即将出版）。

Akinobu Kakigi, Taizo Takeda: "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential" Hearing Research. 116. 34-38 (1998)

Akinobu Kakigi、Taizo Takeda：“人工内淋巴注射到耳蜗管对耳蜗内电位的影响”听力研究。