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Compartmental organization and integration of inputs in the cerebellar nuclei

小脑核的区室组织和输入整合

基本信息

批准号：
16500259
负责人：
SUGIHARA Izumi
金额：
$ 2.37万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

The cerebellum is concerned with the control of various kinds of movements, suggesting precise functional compartmentalization in it. Our previous studies revealed that the aldolase C expression pattern in the cerebellar cortex is tightly related with its functional compartmentalization. Purkinje cell axons, which are the output of the cerebellar cortex, project to the cerebellar nuclei. Compared to the cerebellar cortex, the cerebellar nuclei have not been much studied in terms of the input and output neuronal circuits and the functional compartmentalization.In the cerebellar nuclei, aldolase C is expressed in the terminals of Purkinje cell axons. Therefore, I first reconstructed aldolase C-positive and negative areas in the cerebellar nuclei in the three-dimensional space in the rat. This revealed that the aldolase C-negative and positive areas are each collected in the rostrodorsal, and caudoventral and lateral parts of the cerebellar nuclei.To relate these areas to the olivary projection, I mapped the nuclear collateral terminations of olivocerebellar axons in three-dimensional space in the rat. This revealed very precise topography in the nuclear projection olivary axons. Based on the topography, the functional compartmentalization in the cerebellar cortex is preserved nearly exactly in the cerebellar nuclei. This supported our hypothesis about the functional significance of aldolase C compartmentalization, and suggests that the rostrodorsal part of the cerebellar nuclei (aldolase C-negative) may be concerned with somatosensory-related functions and the caudoventral and lateral part of the cerebellar nuclei (aldolase C-positive) are concerned with other functions.Furthermore, labeled Purkinje cells projections indicated that the closed-loop circuit between the inferior olive and cerebellar cortex and nuclei was generally formed. Thus, precise compartmentalization and integration of neuronal inputs in the cerebellar nuclei were revealed.

小脑与各种运动的控制有关，表明它具有精确的功能分区。我们前期的研究表明醛缩酶C在小脑皮层的表达模式与其功能区隔密切相关。浦肯野细胞轴突是小脑皮层的输出，投射到小脑核。与小脑皮层相比，小脑核在神经元输入输出回路和功能分区方面的研究较少。在小脑核中，醛缩酶C在浦肯野细胞轴突末端表达。因此，我首先在大鼠的三维空间内重建了小脑核内醛缩酶c阳性和阴性区域。这表明醛缩酶c阴性区和阳性区分别聚集在小脑核的背侧、尾腹侧和外侧。为了将这些区域与橄榄叶投影联系起来，我在大鼠的三维空间中绘制了橄榄小脑轴突的核侧支末端。这显示了核投射橄榄轴突的精确地形。从地形图上看，小脑皮层的功能区隔几乎完全保留在小脑核中。这支持了我们关于醛缩酶C区隔化功能意义的假设，并提示小脑核的背侧部分（醛缩酶C阴性）可能与躯体感觉相关的功能有关，而尾侧和外侧部分（醛缩酶C阳性）可能与其他功能有关。此外，标记浦肯野细胞投影显示，下橄榄与小脑皮层和核之间普遍形成闭环回路。因此，揭示了小脑核中神经元输入的精确区隔和整合。