Relationship between the cortical and nuclear functional compartmentalizations in the cerebellum and integration of inputs in the cerebellar nuclei

我们已经揭示了小脑皮质和核的功能区室化的存在下，通过双标记的神经元连接和醛缩酶C，小脑的一个特殊的标记分子的表达。然而，皮层组织，其特征是交替条纹的醛缩酶C阳性和阴性的纵向隔间，是如何反映在小脑核尚未澄清。因此，我们的目的是阐明小脑核的房室组织，这是小脑输出整合的基础，通过详细分析的地形关系，在皮质核浦肯野细胞的投影和橄榄皮质和橄榄攀缘纤维的投影。我们重建浦肯野细胞轴突顺行标记与少量注射生物素葡聚糖胺在大鼠小脑皮质。然后，我们通过免疫组织化学方法鉴定了标记的浦肯野细胞所在的皮质区室。 ...更多信息 醛缩酶C.然后，这些浦肯野细胞轴突的终止区被映射到小脑核。我们发现，浦肯野细胞项目的尾侧和喙部的小脑核的醛缩酶C阳性和阴性车厢在小脑皮质，分别。关于橄榄投射，投射到皮质中的醛缩酶C阳性和阴性隔室的轴突的核侧支分别投射到小脑核的尾侧和喙侧部分。这与上述浦肯野细胞投射模式完全一致。皮质核地形和橄榄皮质核地形几乎完全一致的细节。因此，下橄榄核、小脑皮质和小脑核之间形成了精确的平行环状神经回路。此外，皮层的交替纵向条纹状区室化转变为小脑核的喙尾二分区室化。少

We have been revealing the presence of functional compartmentalization in the cerebellar cortex and nuclei by double-labeling neuronal connection and expression of aldolase C, a special marker molecule of the cerebellum. However, how the cortical organization, which is characterized by the alternate stripes of aldolase C-positive and negative longitudinal compartments, is reflected in the cerebellar nuclei has not been clarified. Therefore, we aimed at elucidating the compartmental organization of the cerebellar nuclei, which underlies integration of the cerebellar output, by analyzing in detail the topographic relationships in the corticonuclear Purkinje cell projection and the olivocortical and olivonuclear climbing fiber projection.We reconstructed Purkinje cell axons by labeling them anterogradely with a small injection of biotinylated dextran amine in the rat cerebellar cortex. We then identified the cortical compartment in which the labeled Purkinje cells were located by immunost … More aing aldolase C. Then the termination areas of these Purkinje cell axons were mapped in the cerebellar nuclei. We found that Purkinje cells project to the caudal and rostral parts of the cerebellar nuclei from the aldolase C-positive and -negative compartments in the cerebellar cortex, respectively. Concerning the olivary projection, nuclear collaterals of the axons that project to the aldolase C-positive and negative compartments in the cortex projected to the caudal and rostral parts of the cerebellar nuclei, respectively. This well agreed to the above Purkinje cell projection pattern. Corticonuclear topography and olivo-cortico-nuclear topography coincided nearly completely in detail. Thus, precise parallel loop-shaped neuronal circuitry was formed among the inferior olive, cerebellar cortex and cerebellar nuclei. Furthermore, the alternate longitudinal stripe-shaped compartmentalization in the cortex was transformed to the rostro-caudal dichotomous compartmentalization in the cerebellar nuclei. Less