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Relationship between the cortical and nuclear functional compartmentalizations in the cerebellum and integration of inputs in the cerebellar nuclei

小脑皮质和核功能区划与小脑核输入整合之间的关系

基本信息

批准号：
18500309
负责人：
SUGIHARA Izumi
金额：
$ 2.63万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500309/
关键词：
cerebellum Purkinje cell cerebellar nuclei cerebellar cortex biotinylated dextran amine aldolase C rat neuroanatomy

项目摘要

We have been revealing the presence of functional compartmentalization in the cerebellar cortex and nuclei by double-labeling neuronal connection and expression of aldolase C, a special marker molecule of the cerebellum. However, how the cortical organization, which is characterized by the alternate stripes of aldolase C-positive and negative longitudinal compartments, is reflected in the cerebellar nuclei has not been clarified. Therefore, we aimed at elucidating the compartmental organization of the cerebellar nuclei, which underlies integration of the cerebellar output, by analyzing in detail the topographic relationships in the corticonuclear Purkinje cell projection and the olivocortical and olivonuclear climbing fiber projection.We reconstructed Purkinje cell axons by labeling them anterogradely with a small injection of biotinylated dextran amine in the rat cerebellar cortex. We then identified the cortical compartment in which the labeled Purkinje cells were located by immunost … More aing aldolase C. Then the termination areas of these Purkinje cell axons were mapped in the cerebellar nuclei. We found that Purkinje cells project to the caudal and rostral parts of the cerebellar nuclei from the aldolase C-positive and -negative compartments in the cerebellar cortex, respectively. Concerning the olivary projection, nuclear collaterals of the axons that project to the aldolase C-positive and negative compartments in the cortex projected to the caudal and rostral parts of the cerebellar nuclei, respectively. This well agreed to the above Purkinje cell projection pattern. Corticonuclear topography and olivo-cortico-nuclear topography coincided nearly completely in detail. Thus, precise parallel loop-shaped neuronal circuitry was formed among the inferior olive, cerebellar cortex and cerebellar nuclei. Furthermore, the alternate longitudinal stripe-shaped compartmentalization in the cortex was transformed to the rostro-caudal dichotomous compartmentalization in the cerebellar nuclei. Less

我们通过双标记神经元连接和醛缩酶 C（小脑的一种特殊标记分子）的表达，揭示了小脑皮质和核中功能区划的存在。然而，以醛缩酶 C 阳性和阴性纵向区室的交替条纹为特征的皮质组织如何反映在小脑核中尚未阐明。因此，我们的目的是通过详细分析皮质核浦肯野细胞投影和橄榄皮质和橄榄核攀登纤维投影的拓扑关系，阐明小脑核的区室组织，这是小脑输出整合的基础。大鼠小脑皮质中的生物素化葡聚糖胺。然后，我们通过免疫醛缩酶 C 识别了标记的浦肯野细胞所在的皮质区室。然后将这些浦肯野细胞轴突的终止区域绘制在小脑核中。我们发现浦肯野细胞分别从小脑皮质中的醛缩酶 C 阳性和阴性区室投射到小脑核的尾部和头侧部分。关于橄榄核投射，投射到皮质中醛缩酶 C 阳性和阴性区室的轴突的核侧支分别投射到小脑核的尾部和头侧部分。这与上述浦肯野细胞投影模式非常一致。皮质核地形和橄榄皮质核地形在细节上几乎完全一致。因此，在下橄榄、小脑皮层和小脑核之间形成了精确的平行环状神经元回路。此外，皮层中交替的纵向条纹状区室化转变为小脑核中的头尾二分区室化。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Relationship of complex spike synchrony bands and climbing fiber projection determined by reference to aldolase C compartments in crus IIa of the rat cerebellar cortex.

参考大鼠小脑皮质小腿 IIa 中的醛缩酶 C 区室确定复杂尖峰同步带与攀爬纤维投影的关系。

DOI：
发表时间：
2007
期刊：
J. Comp. Neurol. 501・1
影响因子：
0
作者：
Ohsawa K;Irino Y;Nakamura Y;Akazawa C Inoue K;Kohsaka S.;Sugihara I.
通讯作者：
Sugihara I.

Functional significance of aldolase C expression pattern in the cerebellar cortex and cerebellar nuclei

小脑皮质和小脑核中醛缩酶 C 表达模式的功能意义

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Sugihara;I.;Shinoda;Y.
通讯作者：
Y.

Molecular, Topographic, and Functional Organization of the Cerebellar Nuclei: Analysis by Three-Dimensional Mapping of the Olivonuclear Projection and Aldolase C Labeling

DOI：
10.1523/jneurosci.1579-07.2007
发表时间：
2007-09
期刊：
The Journal of Neuroscience
影响因子：
0
作者：
I. Sugihara;Y. Shinoda
通讯作者：
I. Sugihara;Y. Shinoda

Identification of aldolase C compartments in the mouse cerebellar cortex by olivocerebellar labeling.

通过橄榄小脑标记鉴定小鼠小脑皮质中的醛缩酶 C 区室。

DOI：
发表时间：
2007
期刊：
J. Comp. Neurol. 500
影响因子：
0
作者：
Ohsawa K;Irino Y;Nakamura Y;Akazawa C Inoue K and Kohsaka S.;Sugihara I.;Keiko Ohsawa;Sugihara I.
通讯作者：
Sugihara I.

Relationship of complex spike synchrony bands and climbing fiber projection determined by reference to aldolase C compartments in crus IIa of the rat cerebellar cortex

参考大鼠小脑皮质小腿 IIa 中的醛缩酶 C 区室确定复杂尖峰同步带与攀爬纤维投影的关系