Protective effect of food omponent against oxidative stress induced by UV irradiation

食品成分对紫外线照射引起的氧化应激的保护作用

• 批准号: 16500505
• 负责人: FUJIWARA Yoko
• 金额: $ 1.98万
• 依托单位: Ochanomizu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500505/
• 关键词: melanogenesis; food component; UV irradaition; oxidative stress; protective effect; メラニン; チロシナーゼ; B16メラノーマ細胞

Melanogenesis is the protective reaction of skin to prevent the cellular DNA damage induced by UV irradiation. As excessive accumulation of melanin is undesirable, however, many studies to inhibit melanogenesis were reported in cosmetic research area. We tied to find out the factors in food to inhibit melanogenesis by oral supplementation.At first, we tried seven kinds of tea extracts from Okinawa to examine the effect of melanin contents in B16 melanoma cells. Most of the tea extracts had no effect except Gettou (Alminia specicosa K.Schum.). The effect of Gettou extract on the activity tyrosinase, a limiting enzyme of melanin synthesis, was investigated. When we tried Gettou in cell-free system using mushroom tyrosinase, Gettou did not inhibit the activity. However, the activity of tyrosinase from B16 melanoma cells incubated with Gettou was lower than cells treated with ascorbic acid. Gettou treatment decreased both mRNA levels and protein levels of tyrosinase in B16 melanoma cells. Gettou had no effect on protein degradation. These results suggested that Gettou treatment decreased the tyrosinase in B16 melanoma cells in transcriptional level.We tried to examine the effect of Gettou by oral supplementation in vivo. Wister-Maples guinea pigs were given water or Gettou tea for five weeks. Their back hair was removed and treated with UV. Although the L^* values of back skin and DOPA positive melanocytes were examined, oral supplementation of Gettou did not show the significant inhibitory effect.

黑素生成是皮肤对紫外线照射引起的细胞DNA损伤的保护性反应。由于黑色素的过度积累是不可取的，然而，在化妆品研究领域，许多研究报道了抑制黑色素生成。为了通过口服补充剂来寻找抑制黑素生成的食物因素，首先，我们尝试了七种来自冲绳的茶提取物，以检查黑素含量对B16黑色素瘤细胞的影响。除Gettou（Alminia specicosa K.Schum.）外，大多数茶提取物没有效果。研究了葛头提取物对黑色素合成的限制酶酪氨酸酶活性的影响。当我们在无细胞系统中使用蘑菇酪氨酸酶尝试Gettou时，Gettou没有抑制活性。然而，酪氨酸酶的活性从B16黑色素瘤细胞孵育Gettou低于细胞与抗坏血酸处理。Gettou治疗降低酪氨酸酶在B16黑色素瘤细胞中的mRNA水平和蛋白水平。Gettou对蛋白质降解没有影响。这些结果表明葛豆处理在转录水平上降低了B16黑色素瘤细胞中的酪氨酸酶。紫藤枫树豚鼠被给予水或葛豆茶五周。他们的背毛被去除并用紫外线处理。虽然检查了背部皮肤和DOPA阳性黑素细胞的L^* 值，但口服Gettou没有显示出显著的抑制作用。