The research of pheromonal memory and reception using immediate early gene Arc.

利用即早基因Arc进行信息素记忆和接收的研究。

• 批准号: 16590138
• 负责人: MATSUOKA Masato
• 金额: $ 1.98万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590138/
• 关键词: vomeronasal organ; pheromone; olfaction; immediate early gene; Arc; memory; mouse; olfactory bulb; 鋤鼻系; シナプス; 神経再生; 最初期遺伝子; ブルース効果

The olfactory system including the vomeronasal system is thought to be a good model for analysis of nerves regeneration. We usually use a nerve transaction for cutting all nerves of olfactory system. However we cannot cut only vomeronasal nerves selectively using this method. To analyze the vomeronasal system functionally, it is important to distinguish between the olfactory and vomeronasal system. On the moment, a new surgical method to. 1it the vomeronasal nerve selectively and reversibly and leave the olfactory system and its nerve fibers intact. For this study, we used a vannis micro dissecting scissors for cutting the vomeronasal nerves at a point just anterior to the accessory olfactory bulb (AOB). We revealed that the number of the OMP positive vomeronasal cell bodies decreased after this surgery, and nerve terminals were completely absent at Day 20-60 without any damages in the olfactory epithelium and the main olfactory bulb (MOB). In addition, we observed the degeneration and regeneration of the vomeronasal axon bundles on the intact MOB after surgery. In a part of experimental animals, the recovery of OMP reactivity was observed after 120 days in both the vomeronasal epithelium and projections within the AOB. Thus, this new method will be a selective complete transaction for vomeronasal system and reversible surgery. It may provide important evidences in vomeronasal regeneration and has the distinct advantage of leaving the olfactory system intact.

嗅觉系统包括犁鼻系统被认为是分析神经再生的一个很好的模型。我们通常使用神经切断术切断嗅觉系统的所有神经。但是，这种方法不能只选择性地切断犁鼻神经。为了从功能上分析犁鼻系统，区分嗅觉系统和犁鼻系统是很重要的。目前，一种新的手术方法。1选择性、可逆性切断犁鼻神经，保留嗅觉系统及其神经纤维的完整。在这项研究中，我们使用vannis微型解剖剪刀在副嗅球(AOB)的正前方切断犁鼻神经。结果显示，术后20~60d，OMP阳性的犁鼻小体数量减少，神经末梢完全消失，嗅上皮和主嗅球(MOB)未见损伤。此外，我们还观察了手术后完整的MOB上的犁鼻轴突束的变性和再生。在部分实验动物中，观察到120天后，犁鼻上皮和AOB内的投射都恢复了OMP的反应性。因此，这种新的方法将是一种选择性的完全性手术，可用于犁鼻系统和可逆性手术。它可能为犁鼻再生提供重要证据，并具有保持嗅觉系统完整的明显优势。

项目成果

最初期遺伝子Arcを用いたフェロモン記憶機構の解析

利用最早的基因Arc分析信息素记忆机制

• 发表时间: 2005
• 期刊: 日本味と匂学会誌 12
• 作者: Li YC;Zhai XY;Ohsato K;Hutamata H;Shimada O;Atsumi S;Ohta T;松岡勝人
• 通讯作者: 松岡勝人

A new surgical approach to the study of vomeronasal system regeneration.

研究犁鼻系统再生的新手术方法。

• DOI: 10.1093/chemse/bjh148
• 发表时间: 2005
• 期刊: Chemical senses
• 影响因子: 3.5
• 作者: Matsuoka,Masato;Norita,Masao;Costanzo,RichardM
• 通讯作者: Costanzo,RichardM

The research of pheromonal memory using immediate early gene Arc.

利用即早基因Arc进行信息素记忆的研究。

• 发表时间: 2005
• 期刊: Japanese Journal of Taste and Smell Research 12(1)
• 作者: Inamura M;Itakura M;Okamoto H;Hoka S;Mizoguchi A;Fukazawa Y;Shigemoto R;Yamamori S;Takahashi M.;Matsuoka M
• 通讯作者: Matsuoka M

Remodeling of reciprocal synapses associated with persistence of long-term memory

• DOI: 10.1111/j.1460-9568.2004.03271.x
• 发表时间: 2004-03-01
• 期刊: EUROPEAN JOURNAL OF NEUROSCIENCE
• 影响因子: 3.4
• 作者: Matsuoka, M;Kaba, H;Ichikawa, M
• 通讯作者: Ichikawa, M