The first animal model for "Hit and Run" theory of oncogenesis by EBV related virus

EBV相关病毒致瘤“肇事逃逸”理论的第一个动物模型

• 批准号: 16590314
• 负责人: HAYASHI Kazuhiko
• 金额: $ 2.3万
• 依托单位: National University Corporation Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590314/
• 关键词: EBV; Herpesvirus papio; Rabbit; Hemophagocytic Syndrome; LPD; T-cell

Epstein-Barr virus associated hemophagocytic syndrome (EBV-AHS) is often associated with fatal infectious mononucleosis or T -cell lymphoproliferative diseases (LPD). To elucidate the pathogenesis of fatal Herpesvirus papio (HVP)-infected LPD and subsequently developed HVP-negative lymphomas observed in HVP-induced rabbit hemophagocytic syndrome, we analyzed sequential examination by ultrasonography (US), CT,CBC, antibody titers, viral quantification, liver biopsy of HVP-induced rabbit LPD and lymphoma development. All of the nine Japanese White rabbits inoculated intravenously with HVP died of fatal LPD or lymphomas. LPD was often accompanied by hemophagocytic syndrome (HPS). Sequential laboratory examinations revealed the increase or decrease of anti-EBV-VCA-IgG titers and viral roads. US or CT examination showed the continuously enlarged splenomegaly. Liver biopsy revealed the transient HVP-EBER-1-positive atypical lymphocyte infiltration. Autopsy examination reconfirmed the development of HVP-EBER-1-negative lymphomas in 2 infected rabbits with long survival and HVP-infected LPD in all cases. Hemophagocytic histiocytosis was observed in the lymph nodes, spleen, bone marrow, or thymus. HVP-DNA was detected in the tissues and peripheral blood from the infected rabbits by PCR or Southern blot analysis. These data suggest that HVP-negative lymphomas developed among HVP-positive LPD may be the good model for "hit and run" oncogenesis theory by herpesvirus.

EB病毒相关噬血细胞综合征(EBV-AHS)常与致死性传染性单核细胞增多症或T细胞淋巴增生性疾病(LPD)有关。为了阐明致死性乳头状疱疹病毒(HVP)感染兔LPD并随后发展为HVP阴性淋巴瘤的发病机制，我们通过超声(US)、CT、血细胞计数、抗体滴度、病毒定量、肝活检等方法对HVP诱导的兔LPD和淋巴瘤的发生进行了分析。静脉接种HVP的9只日本大白兔全部死于致死性LPD或淋巴瘤。LPD常伴有噬血细胞综合征(HPS)。连续的实验室检查显示抗EBV-VCA-Ig G滴度和病毒路径的增加或减少。超声或CT检查显示脾持续增大。肝活检发现一过性HVP-EBER-1阳性的异型淋巴细胞浸润。尸检证实2只长期存活的感染兔出现HVP-EBER-1阴性淋巴瘤，所有病例均为HVP感染的LPD。在淋巴结、脾、骨髓或胸腺可观察到噬血细胞组织细胞增多症。用聚合酶链式反应和Southern印迹杂交检测感染兔的组织和外周血中HVP-DNA。这些数据表明，HVP阳性LPD中发生的HVP阴性淋巴瘤可能是疱疹病毒“打了就跑”致癌理论的良好模型。

项目成果

Expression and intracellular localization of FKHRL1 in mammary gland neoplasms.

FKHRL1 在乳腺肿瘤中的表达和细胞内定位。

• 发表时间: 2004
• 期刊: Acta Med. Okayama 58(4)
• 作者: Gui-Shan J;Kondo E;Miyake T et al.
• 通讯作者: Miyake T et al.

Pathobiological characteristics of the child rabbits infected with Epstein-Barr-like virus (Herpesvirus papio) : comparison with pathological features of HVP-infected adult rabbits

感染Epstein-Barr样病毒（狒狒疱疹病毒）的幼兔的病理生物学特征：与感染HVP的成年兔病理特征的比较

• 发表时间: 2006
• 期刊: J Yonago Med Ass 57-3
• 作者: Machide M;Hashigasako A;Matsumoto K;Nakamura T;Ogahara I
• 通讯作者: Ogahara I

Expression and intracellular localization of FKHRLI in mammary gland neoplasms

FKHRLI在乳腺肿瘤中的表达和细胞内定位

• 发表时间: 2004
• 期刊: Acta Med.Okayama 58・4
• 作者: Nakamura K.;Senda T.;Sato K.;Mori S.;Moriyama M.;Matano S;Jin Gui-Shan et al.
• 通讯作者: Jin Gui-Shan et al.

Thymic myoid cells as a myasthenogenic antigen and antigen-presenting cells

• DOI: 10.1016/j.jneuroim.2004.01.022
• 发表时间: 2004-05
• 期刊: Journal of Neuroimmunology
• 影响因子: 3.3
• 作者: M. Matsumoto;H. Matsuo;T. Oka;T. Fukudome;K. Hayashi;H. Shiraishi;M. Motomura;N. Shibuya;H. Ayabe

Human placenta feeder layers support undifferentiated growth of primate embryonic stem cells

• DOI: 10.1634/stemcells.22-4-433
• 发表时间: 2004-01-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Miyamoto, K;Hayashi, K;Ito, Y
• 通讯作者: Ito, Y