Analysis of speciation process in Liliaceae sensu lato

本文报道了对百合族部分成员生活史特征的比较研究结果，并利用matK进行了沿着分子系统分析。叶绿体基因的转录间隔区和核核糖体DNA（ITS）的内转录间隔区。为了分析百合属及其近缘类群的属间和属内关系，对百合属、野百合属、烛木属、假百合属和贝母属的matK基因和ITS区进行了序列测定，并以日本刺桐和弗吉尼亚苜蓿为外类群。Nomocharis种是百合属的内类群。百合属的三个姐妹类群是假百合属、大百合属和贝母属。本研究结果为百合属近缘类群的系统发育关系提供了新的证据，假百合属和心百合属均为一次结实植物，开花期为8年~ 10年或多年 关于我们 2个亚种，L. mculatum subsp.斑叶草和subsp. dauricum（Ker-Gawl.）原然而，我最近的研究表明，这两个亚种是不同的两个物种，原因有四个。1）形态学;红顶兰地上部茎上有糙硬毛，地上萌发;蝙蝠葛茎上无毛，表现为下胚轴萌发。2)生活史; L. maculatum是一种地上型植物（HETP），L.蝙蝠葛属地上型植物（ETP）。3）染色体：L. maculatum无核仁缢痕，L. dauricum具有高频率的核仁收缩（Noda 1987.）4)分子生物学数据：这3个种在matK基因和rDNA ITS区序列上存在差异。南欧特有的L. bulbiferum从L. dauricum后分为L.卷叶草是日本特有种，为二倍体和三倍体复合种。三倍体兰芝植株含有莱希氏兰芝的部分基因组。马克西莫维奇葱属具有北极第三纪成分的植物以叶扁平为特征。这种形态学特征在本研究中并不符合系统发育的限制。少

The results of comparative studies on the life history characteeristics of selected members of Lileae are reported along with molecular systematic analysis using the matK. of chloroplast genes genes and the internal transcribed spacer region of nuclear ribosomal DNA(ITS). In analyzing the inter-and infra-generic relationships in Lilium and allied taxa, the matK gene and ITS region were sequmenced for Lilium, Nomocharis, Candiocrimun, Notholirion, and Fritillaria species, with Erythronium japoniicum and Medeola virginiana used as outgroup taxa. The Nomocharis species were ingroup taxa of the genus Liliurn. Notholirion, Cardiocrinum, and Fritillaria were demonstrated to be sister groups to Lilium. The present results have shown many new evidence concerning the phylogenetic relationships among the Lilian allied taxa, Genus Notholirion and Cardiocrinum were monocarpic plants and flowering time of these plants took from 8 years 10 to years or more over years.Lilium mculatum sensu lato consi … More sts of two subspecies, L mculatum subsp. maculatum Thunb. and subsp. dauricum(Ker-Gawl.) Hara. However, my recent studies revealed that these two subspecies are different two species with the follwing four reasons. 1)Morphology; L. mculatum has hispid hair on the stem above the ground, and shows epigeal germination; L. dauricum no hair on the stem and shows hypogeal germination. 2)Life history; L. mculatum is a hyo-epigeous type plant(HETP), while L. dauricum is epigeous type plant(ETP). 3)Chromosomes: L. mculatum has no nucleolar constrictions but L. dauricum has nucleolar constrictions in high frequency (Noda 1987.) 4) Moleculer data: these species are different in the sequence patterns of matK gene and ITS of rDNA. The South Europe endemic L. bulbiferum sparated from L. dauricum and after divided to L. mculatum of a endemic species in JapanL.lancifolium was diploid and triploid complex species. Triploid plants of L.lancifoliumcontained a part of genom of L.leichtlinii var. maximowiczii. The plants of having Arcto-Tertiary element in genus Allium is characterized by having flat leaf. This morpholpgical character was not refrected the phylogenetic constraints in the present analysis. Less