Cellular and molecular pharmacological study of the mechanisms underlying the effects of Kanpo medicine.

1)用运动分析仪研究了汉方药物信保通对豚鼠心肌细胞收缩活动的急性作用。Shinbuto（3-5 mg/ml）呈浓度依赖性地使心肌细胞的收缩幅度增加56-369%。但不同条件下得到的结果并不一致。2）Shinbuto长期给药对小鼠心、肾基因表达的影响。心脏中大约50个基因和肾脏中大约100个基因表达出现了明显的变化。其中一个糖尿病相关基因在心脏中表达增加，但在肾脏中表达减少。3）在高血压心力衰竭模型大鼠、自发性高血压大鼠（SHR）中检查了长期给予Shinbuto对患病动物的影响。虽然对照动物（SHR）没有表现出任何心力衰竭的症状，也没有增加利钠肽，但Shinbuto的五种成分中的两种都倾向于抑制SHR的血压，而其他三种则没有表现出对血压的任何影响。信产道各成分均能改变热休克蛋白、肾素-血管紧张素系统、钾离子通道和钙离子通道相关基因的表达。这些基因似乎与高血压有关。正常小鼠服用信产道后，基因表达发生了显著变化，而患病大鼠的基因表达没有发生显著变化。这些结果表明，信产道的作用不仅因物种而异，而且还因病理条件而异。

1) The acute effects of Shinbuto, a Kampo medicine on the contraction activity of guinea-pig cardiac myocytes were studied using a motion analyzer. Shinbuto (3-5 mg/ml) tended to increase the contractile amplitude of myocytes by 56-369% in a concentration dependent manner. However, results obtained under different conditions were inconsistent.2) The effect of long-term Shinbuto administration on heart and renal genes expression in mice. About 50 genes in the heart and about 100 genes expressed in the kidney showed marked changes. One apoptosis-related gene showed increased expression in the heart, but a decrease in the kidney.3) The effect of long-term Shinbuto administration to diseased animals was examined in hypertensive heart failure model rats, spontaneously hypertensive rats (SHR). Although control animals (SHR) did not show any symptoms of heart failure nor an increase in natriuretic peptide, each of two of the five ingredients of Shinbuto individually tended to suppress the blood pressure in SHR, while the other three did not show any effect on blood pressure. Each ingredient of Shinbuto changed the expressions of genes related to heat shock protein, renin-angiotensin system, potassium ion and calcium ion channels. These genes seem to be related to hypertension. Gene expressions changed markedly following Shinbuto administration to normal mice, but did not show any remarkable change in these diseased rats.These findings suggest that the effects of Shinbuto, a Kampo medicine vary not only with species, but also with pathological conditions.