Cellular and molecular pharmacological study of the mechanisms underlying the effects of Kanpo medicine.

汉方医学作用机制的细胞和分子药理学研究。

• 批准号: 16590558
• 负责人: HASEGAWA Junichi
• 金额: $ 2.3万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590558/
• 关键词: Kampo medicine; Pharmacology; model animal; molecular biology; gene expression; 電気生理学; 動物; 心筋細胞

1) The acute effects of Shinbuto, a Kampo medicine on the contraction activity of guinea-pig cardiac myocytes were studied using a motion analyzer. Shinbuto (3-5 mg/ml) tended to increase the contractile amplitude of myocytes by 56-369% in a concentration dependent manner. However, results obtained under different conditions were inconsistent.2) The effect of long-term Shinbuto administration on heart and renal genes expression in mice. About 50 genes in the heart and about 100 genes expressed in the kidney showed marked changes. One apoptosis-related gene showed increased expression in the heart, but a decrease in the kidney.3) The effect of long-term Shinbuto administration to diseased animals was examined in hypertensive heart failure model rats, spontaneously hypertensive rats (SHR). Although control animals (SHR) did not show any symptoms of heart failure nor an increase in natriuretic peptide, each of two of the five ingredients of Shinbuto individually tended to suppress the blood pressure in SHR, while the other three did not show any effect on blood pressure. Each ingredient of Shinbuto changed the expressions of genes related to heat shock protein, renin-angiotensin system, potassium ion and calcium ion channels. These genes seem to be related to hypertension. Gene expressions changed markedly following Shinbuto administration to normal mice, but did not show any remarkable change in these diseased rats.These findings suggest that the effects of Shinbuto, a Kampo medicine vary not only with species, but also with pathological conditions.

1）使用运动分析仪研究了坎波医学对豚鼠心肌细胞收缩活性的急性影响。 Shinbuto（3-5 mg/ml）以浓度依赖性方式将心肌细胞的收缩幅度增加56-369％。然而，在不同条件下获得的结果不一致。2）长期忍者给药对小鼠心脏和肾脏基因表达的影响。心脏中约有50个基因，在肾脏中表达的大约100个基因显示出明显的变化。一个与凋亡相关的基因在心脏中的表达增加，但肾脏的降低。3）在高血压心力衰竭模型大鼠中检查长期忍者对患病动物的影响，自发性高血压大鼠（SHR）。尽管对照动物（SHR）没有显示出任何心力衰竭的症状，也没有表现出纳地酸肽的增加，但申丁的五种成分中的两种单独抑制SHR中的血压，而其他三个成分对血压没有任何影响。 Shinbuto的每种成分都改变了与热休克蛋白，肾素 - 血管紧张素系统，钾离子和钙离子通道相关的基因表达。这些基因似乎与高血压有关。基因表达在正常小鼠中给药后明显变化，但在这些患病的大鼠中没有显示出任何显着变化。这些发现表明，坎波医学的辛布托的影响不仅因物种而有所不同，而且在病理状态下也有所不同。