Effect of renal hyperparathyroidism on the vascular calcification in uremic rats.

肾性甲状旁腺功能亢进对尿毒症大鼠血管钙化的影响。

• 批准号: 16590797
• 负责人: AKIZAWA Tadao
• 金额: $ 1.54万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590797/
• 关键词: Chronic renal failure; Secondary hyperparathyroidism; Ectopic calcification; Vascular calcification; Atherosclerosis; Phosphate; Vitamin D; Calcimimetics; core binding factor-alpha 1(Cbfa1); osteopontin; sodium-dependent phosphate cotransporter

In order to elucidate the effect of secondary hyperparathyroidism and the modulation of parathyroid function on the ectopic vascular calcification in uremic rats, aorta and arterial vessel of the 5/6 nephrectomized rats with severe secondary hyperparathyroidism fed by hyper phosphate and hypo calcium diet were examined. Remarkable ectopic calcification (von Kossa positive staining) was observed in the aorta of experimental uremic rats with secondary hyperparathyroidism, and at the calcified vessel, m-RNA and protein expression of sodium-dependent phosphate co-transporter (Pit-1), core binding factor-alfa 1 (CbFa1) and osteopontin (OPN) were observed. These findings depended on the severity of secondary hyperparathyroidism and hyperphosphatemia and serum calcium and phosphate products. These results suggest that secondary hyperparathyroidism per se stimulates vascular calcification, and promotes the active transport of phosphate into the vessel wall and develops ossification of vascular smooth muscle cell in uremia. Management of calcium, phosphorus and secondary hyperparathyroidism is very important to prevent the vascular calcification and associated atherosclerosis.

为了阐明继发性高甲状腺功能疗法的影响以及甲状旁腺功能的调节对5/6肾切除大鼠的尿毒症大鼠，主动脉和动脉血管的异位血管钙化的调节，并检查了严重的次级甲状腺功能亢进症，由高磷酸盐和低磷酸盐饮食进行了检查。在具有继发性甲状旁腺功能亢进症的实验性尿毒症大鼠的主动脉中观察到了显着的异位钙化（von kossa阳性染色），以及在钙化的血管，M-RNA和蛋白质依赖钠依赖性磷酸磷酸磷酸共磷酸共磷酸盐（PIT-1）的表达，核心结合因子-Alfa 1（cbfa）ways and cbfa和ofn（opn）（opn）。这些发现取决于继发性甲状旁腺功能亢进，高磷酸盐血症以及血清钙和磷酸盐产物的严重程度。这些结果表明，继发性甲状旁腺功能亢进本身会刺激血管钙化，并促进磷酸盐进入血管壁的活性运输，并发展尿素中血管平滑肌细胞的骨化。钙，磷和继发性甲状旁腺功能亢进症的治疗对于防止血管钙化和相关的动脉粥样硬化非常重要。

项目成果

Calcimimetic compound upregulates decreased calcium-sensing receptor expression level in parathyroid glands of rats with chronic renal insufficiency

• DOI: 10.1097/01.asn.0000141016.20133.33
• 发表时间: 2004-10-01
• 期刊: JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
• 影响因子: 13.6
• 作者: Mizobuchi, M;Hatamura, I;Akizawa, T
• 通讯作者: Akizawa, T

The calcimimetic agent KRN 1493 lowers plasma parathyroid hormone and ionized calcium concentrations in patients with chronic renal failure.

拟钙剂 KRN 1493 可降低慢性肾功能衰竭患者的血浆甲状旁腺激素和离子钙浓度。

• 发表时间: 2004
• 期刊: Br J Clin Pharmacol. 57(6)
• 作者: Ohashi N;Togawa A;Akizawa T;Koshikawa S;et al.
• 通讯作者: et al.

How to apply and adapt international guidelines on bone metabolism and disease to the management of Japanese dialysis patients.

如何应用和调整有关骨代谢和疾病的国际指南来管理日本透析患者。

• 发表时间: 2005
• 期刊: Ther Apher Dial. 9(1)
• 作者: Fukagawa M;Akizawa T
• 通讯作者: Akizawa T

Direct maxacalcitol injection into hyperplastic parathyroids improves skeletal changes in secondary hyperparathyroidism

直接注射马沙骨化醇至增生性甲状旁腺可改善继发性甲状旁腺功能亢进症的骨骼变化

• 发表时间: 2006
• 期刊: Kidney Int.2006 70(3)
• 作者: Shiizaki K;Hatamura I;Negi S;Sakaguchi T;Saji F;Kunimoto K;Okamoto M;Imazeki I;Muragaki Y;Akizawa T
• 通讯作者: Akizawa T

Mineral metabolism and haemoglobin concentration among haemodialysis patients in the DOPPS.

DOPPS 中血液透析患者的矿物质代谢和血红蛋白浓度。

• 发表时间: 2005
• 期刊: Nephrol Dial Transplant. 20(5)
• 作者: Kimata N;Akizawa T;et al.
• 通讯作者: et al.