Significance of CD244 expression in functional development of CD8+ cytotoxic T lymphocytes

(1)成人CD 8 + T细胞中约有40-50%为CD 244阳性，且随年龄增长CD 244阳性率逐渐增高，而新生儿CD 8 + T细胞中几乎全部为CD 244阴性。三色流式细胞仪分析CD 244阳性细胞中存在CD 28/CD 62 L阳性或阴性两个亚群，而CD 244阴性的CD 8 + T细胞均为CD 28阳性的CD 62 L阳性。在传染性单核细胞增多症（IM）和麻疹患者中发现了CD 244+细胞的增加。与正常人相比，尤其是CD 28、CD 62 L阳性CD 244阳性CD 8 + T细胞的增加更为显著。(2)分析CD 244阳性和CD 244阴性的CD 8 + T细胞亚群表面抗原和细胞内细胞毒性颗粒蛋白表达的差异。CD 244阴性细胞表面分子表达与未处理细胞基本相同，不表达前体蛋白、颗粒酶B、TIA-1。另一方面，CD 244阳性细胞显示， ...更多信息 记忆细胞表面表型(3)原始T细胞具有丰富的TCR库，但记忆T细胞的TCR库多样性有限，分析了CD 244阳性、CD 244阴性细胞亚群的TCR CDR谱。CD 244阴性细胞在所有TCRVβ中呈高斯分布，但CD 244阳性细胞在许多TCRVβ中呈偏态分布。CD 28阴性细胞和CD 62 L阴性细胞CDR 3谱的复杂性指数较CD 28阳性细胞和CD 62 L阳性细胞明显降低。(4)从正常成人CD 8 +T细胞中分离的CD 244阴性CD 8 + T细胞在体外刺激和孵育。我们证实了在CD 8 + T细胞中从CD 244阴性表型到CD 244阳性表型的转化。CD 244表达在培养早期随CD 3刺激而增加。另一方面，转换为CD 28、CD 62 L-表型需要两到三周，并且进展缓慢。(5)结果显示，CD 244阴性细胞中TREC含量丰富，且TREC含量依次为CD 28 CD 62 L阳性细胞、CD 28 CD 62 L阴性细胞、CD 244阳性细胞。少

(1) About 40-50% adult CD8+ T cell were CD244 positive and CD244 positive fraction increased with aging whereas almost of newborn infant CD8+ T cell was CD 244 negative. CD28/CD62L positive, or negative two subgroup were present in CD244 positive cells when they analyzed three-color flow cytometry, whereas all CD244 negative CD8+ T cells were CD28 positive CD62L positive. Increase of CD244+ cells have been found in patients with infectious mononucleosis (IM) and measles., compared it with a normal subject, and in particular, and increase of CD28,CD62L positive CD244 positive CD8+ T cell were prominent.(2) We analyzed the difference of expression of surface antigen and intracellular cytotoxic granular proteins in CD244-positive, and CD244-negative CD8+ T cell subgroup. CD244-negative cells showed surface phenotype almost same as naive cell from all surface molecule expression and did not show perform, granzyme B, TIA-1 expression either. On the other hand, CD244-positive cells showed me … More mory cell surface phenotype.(3) Naive T cell have TCR repertoire with full of diversity, but T cell repertoire diversity was expected to be limited in memory-T cells, and TCR CDR spectrum were analyzed in each CD244-positive, CD244-negative cell subgroup. CD244 negative cells presented Gaussian distribution in all TCRVβ, but CD244-positive demarcation showed a skewed pattern in many TCRVβ. In addition, complexity index of CDR3 spectra in CD28-negative cell or CD62L negative cell decreased more than that in CD28 positive cells, or CD62L positive cell.(4) CD244-negative CD8+ T cell separated from normal adult CD8+T cells were stimulated and incubated, in vitro. We confirmed conversion from CD244-negative to CD244-positive phenotypte in CD8+ T cells. CD244 expression were increased with CD3 stimulation at early stage of culture. On the other hand, switch to CD28,CD62L- phenotype took two or three weeks and progressed in slowly.(5) We measured TREC content in each cell fractionation to quantify degree of mitosis in functional differentiation phase.CD 244 negative cells showed abundant TREC content, and the TREC content decreased in order of CD28CD62L positive CD244-positive cells, CD28/CD62L negative CD244-positive cell populations. Less