Enhancement of Immunological Activity and drug Uptake to Tumor by Mild-Hyperthermia.

一般来说，热疗（HT）治疗在42.5℃的温度下进行1小时。认为39 ~ 40℃轻度高温对体外系统无抗肿瘤作用。但在本研究中，我们发现即使在体内温度为39℃~ 40℃时，由于免疫状态的激活，也有抗肿瘤作用。将肿瘤细胞移植于小鼠右大腿。右大腿在39℃~ 40℃的控制水浴中加热1小时。给予轻度高温后，NK细胞活性、白细胞、淋巴细胞、粒细胞和单核细胞的数量与预热条件下相比短暂增加。轻度高温治疗后，C3H小鼠的SCC-VII肿瘤生长延迟，肿瘤植入失败。化疗联合轻度ht由于增加了肿瘤组织对药物的摄取而增强了抗肿瘤作用。在41℃以上的温度下，持续30 min，并联合顺铂（7mg/kg， IP）作为抗癌药物。但联合轻度ht组肿瘤组织中药物浓度较单用药物组高7倍。与轻度th联合化疗时，单热或单药治疗均显著增加肿瘤生长延迟。羟色胺具有增强细胞膜通透性的作用。这些结果表明，HT具有血管扩张增加血流量的作用，并且由于药物离子通道功能的降低而使细胞膜转变为高通透性。因此，在化疗中使用温和的ht可以减少药物的使用，在临床上可以减少肿瘤治疗的副作用。由于没有任何副作用，轻度HT不仅对恶性肿瘤的治疗有效，而且对丙型肝炎、艾滋病等与免疫紊乱有关的疾病的治疗也很有效。热疗有血管扩张的作用，增加了受热区域的血流量。少

In general, hyperthermia (HT) therapy is performed at a temperature of 42.5℃ for 1 hour. It is believed that mild HT at 39℃ to 40℃ brings no anti-tumor effects in the in vitro system. But in the present study, we found that there were anti-tumor effects even when at a temperature of 39℃ to 40℃ in vivo due to activation of immunological status. The tumor cells were transplanted on the right thigh of mice. HT was brought by giving a heat to the right thigh in a controlled water bath at 39℃ to 40℃ for 1 hour. After giving the mild HT, NK cells activity and the numbers of white blood cells, lymphocyte cells, granulocyte, and monocyte increased transiently in comparison with those in the pre-heated condition. A delay in SCC-VII tumor growth in C3H mice and a failure in the implantation of tumor were also observed after the mild HT. The chemotherapy combined with mild-HT were increased the anti-tumor effect due to the increase of drug uptake into the tumor tissues. HT were carried out at 41 … More degree for 30 min and combined with cisplatin (7mg/kg, IP) as anti-cancer drug. But combined with mild-HT were 7-times of drug concentration higher than drug alone group in the tumor tissues. When combined with mild-TH and chemotherapy, tumor growth delay were significantly increased combined with heat alone or drug alone treatment. The HT has effect of enhanced the permeability of the cell membrane. These results suggest that HT has effect of increase of blood flow due to the vasodilatation and cell membrane were changed to high permeability of the drug due to the reduced the function of ion channels. Therefore, the chemotherapy may reduced the drugs using mild-HT and it can be reduced the side effects for treatment of tumor on clinically. Mild HT seems to be a useful and effective auxiliary therapy which is not only effective for the treatment of malignant tumors but also for the treatment of diseases related to immunological disturbances such as hepatitis type C, AIDS etc because of no any side effects. And HT has effects of vessel dilatation and increased a blood flow in the heating areas. Less