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Effect of Growth factor on intrauterine growth retardation and factor for growth factor production

生长因子对子宫内生长迟缓的影响和生长因子产生因子

基本信息

批准号：
16591690
负责人：
YAMAMOTO Tatsuo
金额：
$ 1.41万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591690/
关键词：
IUGR Growth factor Pregnancy induced hypertension VEGF PLGF sFlt-1 real time PCR 妊娠高血圧症候 Flt-1 絨毛癌細胞培養細胞株 JEG-3 定量的PCR法胎盤 sFLt-1 絨毛妊娠高血圧

项目摘要

1.Placental Growth Factor(PIGF) is a growth factor originated from placenta belong to VEGF family. VEGF and PLGF may play a role of proliferation of trophoblast. The sVEGFR1 is soluble receptor for VEGF and PLGF. The sVEGFR1 suppresses functional activity of VEGF and PLGF. We measured the levels of free VEGF, PLGF and sVEGFR1 in preeclamptic sera. The levels of free VEGF and PLGF in preeclamptic sera were lower than those in normal pregnant serum. However the levels of sVEGFR1 in preeclamptic sera were higher than that in normal pregnant serum.2.To elucidate the pathophysiology of preeclampsia, we examined the effects of preeclamptic sera on the production of VEGF, PLGF and sVEGFR1 from cultured choriocarcinoma cell line. The levels of free VEGF in preeclamptic sera were lower than those in normal pregnant serum. The levels of P1GF had not significant change between them. However the levels of sVEGFR1 in preeclamptic sera were higher than that in normal pregnant serum. Preeclamptic ser … More a may play a role of increased production of sVEGFR1 keeping decreased levels of free VEGF or P1GF in preeclampsia.3. To elucidate the pathophysiology of pregnancy induced hypertension (PIH), we examined the expression of VEGF, PLGF and sVEGFR1 mRNA in placenta taken from PIH patients. The expression of PLGF mRNA in PIH placenta were significantly lower than those in normal pregnancies (p=0.014). The expression of VEGF and sVEGFR1 mRNA in PIH placenta had wide variation and were not different from those of normal pregnancies. However, the positive correlation was suggested between the expression of VEGF and sVEGFR1 (r=0.81).4. We studied the relationships between hypertensive disorders of pregnancy and the human Renin gene and/or the human coagulation factor XI gene. We detected the association between them.5. We evaluated an anti-phosphatidylserine-prothrombin antibody in patients with recurrent abortion and preeclampsia and detected this antibody in them.6. In order to know the comlement activation, the changes of compliment were evaluated in recurrent abortion and pregnancy loss patients with antiphospholipid antibody(APA) positive. The comlement activation was demonstrated in recurrent abortion and pregnancy loss with antiphospholipid antibody positive. Less

1.胎盘生长因子(PIGF)是一种来源于胎盘的生长因子，属于血管内皮生长因子家族。VEGF和PLGF可能在滋养层细胞的增殖中起作用。SVEGFR1是血管内皮细胞生长因子和血小板衍生生长因子的可溶性受体。SVEGFR1抑制血管内皮生长因子和血小板衍生生长因子的功能活性。检测子痫前期患者血清中游离VEGF、PLGF和sVEGFR1的水平。子痫前期患者血清中游离VEGF和PLGF水平均低于正常妊娠血清。2.为阐明子痫前期的病理生理机制，我们观察了子痫前期血清对培养的绒毛癌细胞产生血管内皮生长因子、血小板源性生长因子和可溶性血管内皮生长因子受体的影响。子痫前期患者血清中游离血管内皮生长因子水平低于正常孕妇血清。两组间P1GF水平无明显变化。而子痫前期患者血清中sVEGFR1水平明显高于正常孕妇血清。先兆子痫Ser…更多的a可能在增加sVEGFR1的产生，维持先兆子痫患者体内游离VEGF或P1GF水平的降低中发挥作用。为探讨妊娠高血压综合征(PIH)的病理生理机制，我们检测了妊娠高血压综合征(PIH)患者胎盘组织中血管内皮生长因子(VEGF)、血小板源性生长因子(PLGF)和血管内皮生长因子受体1(SVEGFR1)的表达。妊高征组胎盘组织中PLGF mRNA的表达显著低于正常妊娠组(p=0.014)。妊高征组胎盘组织中VEGF和sVEGFR1mRNA的表达差异较大，与正常妊娠组无明显差异。而VEGF和sVEGFR1的表达呈正相关(r=0.81)。我们研究了妊娠期高血压疾病与人肾素基因和/或人凝血因子XI基因的关系。我们检测到了它们之间的联系。我们评估了一种抗磷脂酰丝氨酸-凝血酶原抗体在反复流产和子痫前期患者中的存在，并在他们身上检测到了这种抗体。为了解补体激活情况，对抗磷脂抗体(APA)阳性的反复流产和妊娠丢失患者的补体功能变化进行了评价。抗磷脂抗体阳性的反复流产和妊娠丢失患者均表现为结合激活。较少