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Resolution of quorum-sensing systems in dental plaque biofilm and development of the method that prevents the plaque formation by the control of them

牙菌斑生物膜中群体感应系统的解析以及通过控制它们来防止牙菌斑形成的方法的开发

基本信息

批准号：
16592050
负责人：
HOSHINO Tomonori
金额：
$ 2.24万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Dental plaque is the biofilm which is formed on the tooth surface, and is a protective barrier for the bacteria against the change of the environment, the antibiotic, and the host immune response. First this biofilm is formed by the extracellular polysaccharide (glucan), which the mitis group streptococci produce from sugar, and then is reinforced by the adhesive and water-insoluble glucan, which the mutans group streptococci produce. Dental plaque biofilm plays an important role in the caries formation. In this biofilm, the plaque bacteria communicate with other bacteria by the auto-inducer like hormone. By this communication system that is commonly known as quorum-sensing, the bacterial population density in the dental plaque is thought to be kept constant, and the bacterial mass against the host immune system is constructed.In the present study, to solve the quorum-sensing system in the dental plaque, we developed the method to identify the oral streptococci, which were the members of this biofilm. This method could rapidly identify the streptococci which were dominant and occupied 60% in the oral flora. We published the paper about it entitled "Method for rapid identification of oral streptococci by PCR using 16S-23S ribosomal RNA intergenic spacer". On the other hand, we investigated the longitudinal study of oral flora against the 2 predentate infants and the cross-sectional study of oral flora against the 40 nursery school students (aged 1 to 4 years old). As a result, we demonstrated that the rate of alpha-hemolytic bacteria in oral flora decreased by the inhabitation of the mutans streptococci, suggested that the quorum-sensing system between mutans streptococci and alpha-hemolytic bacteria existed. We had submitted the paper about this entitled "Establishment of S.mutans Change Salivary Bacterial ratio".Our future study is to identify auto-inducer produced between mutans streptococci and alpha-hemolytic bacteria.

牙菌斑是在牙齿表面形成的生物膜，是细菌抵御环境变化、抗生素和宿主免疫反应的保护屏障。首先，该生物膜由细胞外多糖（葡聚糖）形成，该细胞外多糖（葡聚糖）是由糖类链球菌产生的，然后由变形链球菌产生的粘合剂和水不溶性葡聚糖增强。牙菌斑生物膜在龋齿的形成中起着重要作用。在这种生物膜中，牙菌斑细菌通过自诱导剂（如激素）与其他细菌进行交流。通过这种通常被称为群体感应的通信系统，牙菌斑中的细菌种群密度被认为保持恒定，并构建了针对宿主免疫系统的细菌群。在本研究中，为了解决牙菌斑中的群体感应系统，我们开发了识别口腔链球菌的方法，口腔链球菌是该生物膜的成员。该方法可快速鉴定口腔菌群中占优势的链球菌，占60%。我们发表了题为“Method for rapid recognize oforal streptococci by PCR using 16S-23S ribosomal RNA intergenic spacer”的论文。另一方面，我们调查了针对2名产前婴儿的口腔菌群的纵向研究和针对40名幼儿园学生（年龄1至4岁）的口腔菌群的横断面研究。结果表明，口腔菌群中α溶血菌的比例因变形链球菌的栖息而降低，表明变形链球菌和α溶血菌之间存在群体感应系统。我们已经提交了题为“改变唾液细菌比率的变形链球菌的建立”的论文。我们未来的研究是鉴定变形链球菌和α-溶血菌之间产生的自诱导子。