Pursuit of multiplication control of the periodontosis bacillus by a new molecule target cure, and the possibility as the anti-periodontosis medicine

反义法用于牙周病病原菌的检测尚无报道。在本研究中，我们利用不受RNase影响的人工核酸PNA对这些致病菌进行反义检测。反义PNA与牙周炎的病原菌牙龈卟啉单胞菌发生反应。因此，我们没有获得预期的反义作用，这是因为牙龈卟啉菌较强的肽酶活性水解了膜转运引导蛋白或肽键构建的PNA主体。另一方面，作为龋齿致病菌的变形链球菌的反义PNA倾向于抑制靶向gtfB基因的表达，但没有显示出显著差异。在我们未来的研究中，有必要研究促进PNA穿透细菌膜的肽序列和有效抑制目标基因表达的反义PNA序列。

There has been no report that antisense method is applied to causative bacteria of the periodontal disease. In this study, we investigated whether antisense method was available for those causative bacteria by using PNA that was the artificial nucleic acid and was not affected by RNase. Antisense PNA was reacted with Porphyromonas gingivalis that was causative organism of the periodontitis that was one of the periodontal diseases. As a result, we did not obtain the expected antisense effect because the strong peptidase activity of P. gingivalis hydrolyzed the membrane transport guidance protein or the main body of PNA constructed of peptide bonds. On the other hand, antisense PNA against Streptococcus mutans that was carious causative bacteria tended to suppress the targeted gtfB gene expression although the significant difference was not shown. In our future study, it is necessary to investigate the peptide sequence that facilitate for PNA to penetrate bacterial membrane and the antisense PNA sequence that inhibit the targeted gene expression efficiently.