Pursuit of multiplication control of the periodontosis bacillus by a new molecule target cure, and the possibility as the anti-periodontosis medicine

新分子靶点治疗对牙周病杆菌增殖控制的探索以及作为抗牙周病药物的可能性

• 批准号: 21659477
• 负责人: HOSHINO Tomonori
• 金额: $ 2.08万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659477/
• 关键词: Porphyromonas gingivalis; Streptococcus mutans; 分子標的治療; PNA; ペプチド核酸(PNA); アンチセンス法; 膜透過型ペプチド; 歯周病; 齲蝕; Aggregatibacter actinomycetemcomitans

There has been no report that antisense method is applied to causative bacteria of the periodontal disease. In this study, we investigated whether antisense method was available for those causative bacteria by using PNA that was the artificial nucleic acid and was not affected by RNase. Antisense PNA was reacted with Porphyromonas gingivalis that was causative organism of the periodontitis that was one of the periodontal diseases. As a result, we did not obtain the expected antisense effect because the strong peptidase activity of P. gingivalis hydrolyzed the membrane transport guidance protein or the main body of PNA constructed of peptide bonds. On the other hand, antisense PNA against Streptococcus mutans that was carious causative bacteria tended to suppress the targeted gtfB gene expression although the significant difference was not shown. In our future study, it is necessary to investigate the peptide sequence that facilitate for PNA to penetrate bacterial membrane and the antisense PNA sequence that inhibit the targeted gene expression efficiently.

目前还没有将反义方法应用于牙周病致病菌的报道。在本研究中，我们利用不受 RNase 影响的人工核酸 PNA 来研究反义方法是否适用于那些致病菌。反义PNA与作为牙周病之一的牙周炎的致病菌的牙龈卟啉单胞菌反应。结果，我们没有获得预期的反义效果，因为牙龈卟啉单胞菌强烈的肽酶活性水解了膜转运引导蛋白或由肽键构成的PNA主体。另一方面，针对作为龋齿致病菌的变形链球菌的反义PNA倾向于抑制目标gtfB基因表达，尽管没有显示出显着差异。在我们未来的研究中，有必要研究促进PNA穿透细菌膜的肽序列和有效抑制靶基因表达的反义PNA序列。

项目成果

Horizontal Transmission of Glucosyltransferase Genes in Streptococcus mutans

变形链球菌中葡萄糖基转移酶基因的水平传播

• 发表时间: 2012
• 作者: Hoshino;T.;Kawabata;S.;Fujiwara;T.
• 通讯作者: T.

Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans

• DOI: 10.1128/cvi.05041-11
• 发表时间: 2011-09-01
• 期刊: CLINICAL AND VACCINE IMMUNOLOGY
• 作者: Hoshino, Tomonori;Kondo, Yoshio;Fujiwara, Taku
• 通讯作者: Fujiwara, Taku

Novel Epitopic region of Glucosyltransferase-B from Streptococcus mutans MT8148

变形链球菌 MT8148 葡萄糖基转移酶 B 的新表位区

• 发表时间: 2010
• 作者: Tomonori Hoshino;Kan Saito;Taku Fujiwara
• 通讯作者: Taku Fujiwara

Novel Epitopic region of Glucosyltransferase-B from Streptococcusmutans MT8148

变异链球菌 MT8148 的葡萄糖基转移酶 B 的新表位区

• 发表时间: 2010
• 作者: Tomonori Hoshino;Kan Saito;Take Fujiwara
• 通讯作者: Take Fujiwara

Establishment of Streptococcus mutans in infants induces decrease in the proportion of salivaryα-haemolytic bacteria

婴儿体内变形链球菌的建立导致唾液α-溶血菌比例下降

• DOI: 10.1111/j.1365-263x.2011.01183.x
• 发表时间: 2012
• 期刊: International Journal of Paediatric Dentistry
• 影响因子: 3.8
• 作者: Mamoru Kawaguchi;* Tomonori Hosohino;Takashi Ooshima;Taku Fujiwara
• 通讯作者: Taku Fujiwara