Differentiation Mechanism of Embryonic Stem Cells into Neural Stem Cells

胚胎干细胞向神经干细胞的分化机制

• 批准号: 17500214
• 负责人: NAKAYAMA Takashi
• 金额: $ 2.24万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500214/
• 关键词: Embryonic stem cell; Astrocyte; Neural stem sphere; Neural stem cell; Neuron; 胚性幹細胞; アストログリア; 分化; 遺伝子発現

Neural stem cells differentiate from embryonic stem (ES) cells via formation of neural stem spheres (NSSs) under free-floating conditions in astrocyte-conditioned medium (ACM). During subsequent culture of the NSSs on an adhesive substrate with fibroblast growth factor (FGF)-2, the neural stem cells are induced to migrate onto the substrate, resulting in an expansion of the cell number. Furthermore, almost all these cells can be differentiated into functional neurons by changing the medium from Neurobasal B-27 to ACM, suggesting that differentiation of the ES-cell-derived neural stem cells into neurons requires some instructive factors present in ACM. In addition, these ES-cell-derived neural stem cells can be differentiated almost exclusively into astrocytes by withdrawing FGF-2 from the medium without any additional humoral factors. This astrocytegenesis does not require any additional instructions in contrast to neurogenesis. Our results indicate that differentiation along the astrocyte lineage is a default state of ES-cell-derived neural stem cells

神经干细胞通过在星形胶质细胞条件培养基（ACM）中的自由漂浮条件下形成神经干球（NSS）而从胚胎干（ES）细胞分化。在随后的培养过程中，在具有成纤维细胞生长因子（FGF）-2的粘性基质上的NSS，神经干细胞被诱导迁移到基质上，导致细胞数量的扩增。此外，通过将培养基从Neurobasal B-27改变为ACM，几乎所有这些细胞都可以分化为功能性神经元，这表明ES细胞来源的神经干细胞分化为神经元需要ACM中存在的一些指导性因子。此外，这些ES细胞衍生的神经干细胞可以通过从培养基中提取FGF-2而几乎完全分化为星形胶质细胞，而无需任何额外的体液因子。与神经发生不同，星形胶质细胞的发生不需要任何额外的指令。我们的研究结果表明，分化沿着星形胶质细胞谱系是一个默认状态的胚胎干细胞衍生的神经干细胞

项目成果

Embryonic Stem Cell Protocols, Vol II : Differentiation Models, Sec.Ed. (Ed.by Turksen K)

胚胎干细胞协议，第二卷：分化模型，第二部分。

• 发表时间: 2006
• 作者: Nakayama;T.
• 通讯作者: T.

Directed differentiation of embryonic stem cell-derived neural stem cells into astrocytes

胚胎干细胞来源的神经干细胞定向分化为星形胶质细胞

• 发表时间: 2005
• 期刊: J Neurochem 94 S2
• 作者: Sawamoto N;Honda M;Hanakawa T;et. al.;Nakayama T
• 通讯作者: Nakayama T

Gene expression during differentiation from primate embryonic stem cells into neural cells by Neural Stem Sphere method.

通过神经干球方法从灵长类胚胎干细胞分化为神经细胞期间的基因表达。

• 发表时间: 2006
• 期刊: Neurosci Res 55
• 作者: Hanakawa T;Dimyan M;Hallett M.;Sai T
• 通讯作者: Sai T

Effects of astrocyte-derived factor and cell-cell communication on uni-directional differentination from mouse embryonic stem cells into neural cells.

星形胶质细胞衍生因子和细胞间通讯对小鼠胚胎干细胞单向分化为神经细胞的影响。

• 发表时间: 2006
• 期刊: Neurosci Res 55
• 作者: Namiki C;et. al.;Tasuno R;Otsu M
• 通讯作者: Otsu M

Changes in gene expression during differentiation from embryonic stem cells into neurons by neural stem sphere method.

通过神经干球法从胚胎干细胞分化为神经元过程中基因表达的变化。

• 发表时间: 2005
• 期刊: J Neurochem 94
• 作者: Mikuni N;et. al.;Nakayama T;花川 隆;Sai T
• 通讯作者: Sai T