Production of volatile organic compounds (VOCs) by marine bacteria

海洋细菌产生挥发性有机化合物（VOC）

• 批准号: 17510015
• 负责人: HASHIMOTO Shinya
• 金额: $ 2.11万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17510015/
• 关键词: methyl iodide; marine bacterium; Alteromonas macleodii; GC-MS; Alteromonas Macleodii; Vibrio splendidus

We evaluated the potential of a marine bacterium, Alteromonas macleodii, to produce CH_3I. Bacterium was incubated at temperatures (10,15,20,25,30℃) in marine broth 2216 with or without 1 mmol/L of potassium iodide. From 15 to 30℃, the higher the culture temperature, the more rapid the proliferation rate. Proliferation rate was not affected by potassium iodide. CH_3I was produced between 15 and 30℃ with potassium iodide. Optimum temperature for CH_3I production was 25℃. A. macleodii was also incubated at 25℃ in medium spiked with potassium iodide from 0.1 to 1000μmol/L. Results showed that the greater the concentration of spiked iodide, the more CH_3I was produced. Notably, production of CH_3I strongly increased at over 10μmol/L of potassium iodide. At the concentration of iodine in surface seawater (about 0.4μmon/L), the CH_3I produced was estimated to be about 0.2μmol/L. This suggested that marine bacteria would produce about 2-20 % of CH_3I in surface seawater (the total number of bacteria in medium was 100-1000 times higher than that seen in surface seawater).

我们评估了海洋细菌，Alteromonas macleodii，产生CH_3I的潜力。细菌在海洋肉汤2216中培养，温度为10，15，20，25，30℃，加或不加1 mmoL/L的碘化钾。在15~30℃范围内，培养温度越高，增殖越快。碘化钾对细胞增殖率无明显影响。用碘化钾在15~30℃之间生成CH3I。产甲烷的最适温度为25℃。在25℃的条件下，添加0.1~1000μ/L的碘化钾，结果表明，添加的碘化钾浓度越大，甲烷的生成量越大。值得注意的是，当碘化钾的摩尔/L超过10μ时，CH_3I的生成量显著增加。在表层海水中碘的浓度(约0.4molmoL/L)下，产生的CH_3I约为0.2moL/L，这表明海洋细菌产生的CH_3I约占表层海水中CH_3I的2-20%(培养液中的细菌总数是表层海水中细菌总数的100-1000倍)。