High accuracy function analysis of mitochondria with organelle adsorption method

目的：虽然细胞器的功能非常重要，但其功能测定方法的发展还不够。细胞器吸附法是一种测量吸附在碱上的细胞器活性的新方法。其特点是单个细胞器的测量和从细胞器中快速分离代谢物。在这项研究中，我们用细胞器吸附法测量线粒体行为。结果与讨论：我们测量了1)解偶联蛋白2 （UCP2）、2)亲环蛋白D、3)神经节苷脂GD3、4)Ca2+、5)呼吸底物对线粒体行为的影响，并在国际期刊发表了4篇原创论文，在国内期刊发表了1篇综述。结果表明：(1)UCP2和亲环蛋白D保护线粒体免受氧化应激损伤；(2)GD3促进线粒体产生活性氧并诱导线粒体逐渐去极化；(3)ADP、ATP和Mg2+的存在稳定了细胞内线粒体膜电位；(4)Ca2+诱导去极化线粒体肿胀而非极化线粒体。这些结果为我们提供了线粒体行为的重要信息。

Purpose:Developments of the method for measuring organelles' functions are not enough, although their functions are very important. Organelle adsorption method is a newly developed method which enables us to measure the activity of organelles adsorbed on the base. The characteristics are the measurements of individual organelles and the quick separation of the metabolites from the organelle. In this study, we measured mitochondrial behaviors with organelle adsorption method. Results and Discussions:We measured the effects of 1) the uncoupling protein 2 (UCP2), 2) cyclophilin D,3) ganglioside GD3, 4) Ca2+, 5) respiration substrates on mitochondrial behaviors, and published 4 original papers in international journal and one review in domestic journal. The results are 1) UCP2 and cyclophilin D protect mitochondria from oxidative stress-induced damage, 2) GD3 promotes the generation of reactive oxygen species from mitochondria and induces the gradual depolarization, 3) mitochondrial membrane potential are stabilized in the cell by the presence of ADP, ATP and Mg2+, 4) Ca2+ induced the swelling of depolarized mitochondria not but polarized mitochondria. These results provide us the important information on mitochondrial behaviors.