Assessment of the health effects of fumes generated from frying oil by DNA micro array

通过 DNA 微阵列评估煎炸油产生的烟雾对健康的影响

• 批准号: 17580102
• 负责人: FUJIMOTO Kenshiro
• 金额: $ 2.18万
• 依托单位: Koriyama Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580102/
• 关键词: frying oil; triacylglycerol; diacylglycerol; fume; volatile aldehydes; cyototoxicity; gene expression; micro array; 酸価; 揮発性成分; DNAマイクロアレイ; 毒性

The health effects of the fumes generated from potato frying were assessed by the incubation with cultivated rat hepatocytes and the DNA microarray technology. Potatoes were 'fried using the salad oil (TAG) or the diacylglycerol-rich oil (DAG) and the fumes were collected in the cold trap to incubated with the rat hepatocytes or introduced to a glass vessel-in which mice were kept to assess the effect on gene expression-pattern in the liver. Chemical analysis and cytotoxicity assessment showed that the major cytotoxic substances could be volatile aldehydes in both oils. Expression of many genes in the liver such as metallothionein and lipin were commonly accelerated with disposure to the fumes from two frying oils, but the expression of some genes related to interferon was accelerated more remarkably by the fume from DAG than that from TAG.

利用培养的大鼠肝细胞和DNA微阵列技术，对煎炸马铃薯产生的烟雾对健康的影响进行了评估。土豆用色拉油（TAG）或富含二酰基甘油的油（DAG）油炸，烟雾被收集在冷阱中与大鼠肝细胞一起孵卵，或者被引入一个玻璃容器中，老鼠被保存在玻璃容器中，以评估对肝脏基因表达模式的影响。化学分析和细胞毒性评价表明，两种精油的主要细胞毒性物质可能是挥发性醛类物质。两种煎炸油的烟雾通常会加速肝脏中金属硫蛋白和脂素等许多基因的表达，但一些与干扰素相关的基因的表达被DAG的烟雾比TAG的烟雾更显著地加速。

项目成果

Deterioration of diacylglycerol-and triacylglycerol-rich oils during frying potatoes

富含二酰基甘油和三酰基甘油的油在煎炸土豆过程中变质

• 发表时间: 2005
• 期刊: European Journal of Lipid Science and Technology 107(2)
• 作者: Li;Chang Mo
• 通讯作者: Chang Mo

DNA microarray technology evaluation of the effects of frying oil fumes on gene expression in the mouse liver.

DNA微阵列技术评估煎炸油烟对小鼠肝脏基因表达的影响。

• 期刊: J. Cookery Sci. Jpn. (in preparation)
• 作者: Fujimoto;K.;Watanabe;N.;Hiroi;M.;Sakajo;S.
• 通讯作者: S.

Cytotoxicity evaluation of oil fumes in rat hepatocytes using a modelsystem for deep-fat frying

使用油炸模型系统评估大鼠肝细胞中油烟的细胞毒性

• 发表时间: 2008
• 期刊: Food Sci.Technol.Res. 14
• 作者: Kimura;F.;Li;CM.;Fndo;Y.;Fujimoto;K.
• 通讯作者: K.

Cytotoxicity evaluation of oil fumes in rat hepatocytes using a model system for deep-fat frying.

使用油炸模型系统评估大鼠肝细胞中油烟的细胞毒性。

• 发表时间: 2008
• 期刊: Food Sci. Technal. Res. 14(in press)
• 作者: Kimura;F.;Li.;CM.;Endo;Y.;Fujimoto;K.
• 通讯作者: K.

Deterioration of diacyl-and triacylglycerol-rich oils during frying potatoes.

煎炸土豆时富含二酰基和三酰基甘油的油会变质。

• 发表时间: 2005
• 期刊: Eur. J. Lipid Sci. Technol. 107
• 作者: Li;C.M.;Kimura;F.;Endo;Y.;Maruyama;C.;Fujimoto;K.
• 通讯作者: K.