Assessment of the health effects of fumes generated from frying oil by DNA micro array

通过培养的大鼠肝细胞孵育和DNA芯片技术，评价了油炸土豆产生的烟雾对人体健康的影响。用色拉油(TAG)或富含二酰甘油的油(DAG)炸土豆，收集油烟与大鼠肝细胞孵化或引入玻璃容器--在玻璃容器中评估对小鼠肝脏基因表达模式的影响。化学分析和细胞毒性评估表明，两种油中的主要细胞毒性物质都可能是挥发性醛。处理后，肝脏中的许多基因，如金属硫蛋白和脂类，通常会被两种油烟加速表达，但DAG的油烟对干扰素相关基因的表达的促进作用比TAG更明显。

The health effects of the fumes generated from potato frying were assessed by the incubation with cultivated rat hepatocytes and the DNA microarray technology. Potatoes were 'fried using the salad oil (TAG) or the diacylglycerol-rich oil (DAG) and the fumes were collected in the cold trap to incubated with the rat hepatocytes or introduced to a glass vessel-in which mice were kept to assess the effect on gene expression-pattern in the liver. Chemical analysis and cytotoxicity assessment showed that the major cytotoxic substances could be volatile aldehydes in both oils. Expression of many genes in the liver such as metallothionein and lipin were commonly accelerated with disposure to the fumes from two frying oils, but the expression of some genes related to interferon was accelerated more remarkably by the fume from DAG than that from TAG.