Development of needle-type biosensor system for monitoring of fish health
开发用于监测鱼类健康的针型生物传感器系统
基本信息
- 批准号:17580180
- 负责人:
- 金额:$ 1.73万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2006
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Mass outbreaks of pathogenic bacterial infections have killed large quantities of fish in recent years, causing great financial damage to fish farms. Periodic checks of fish health and detection of abnormalities as quickly as possible are thus necessary at fish farms. Several studies have shown blood glucose levels closely correlate to the level of stress in fish and represent the state of respiratory or nutritional disturbance. Recent studies have also clarified that decreased levels of blood cholesterol represent a useful indicator of reduced resistance to bacterial infection. In this manner, determination of blood glucose and cholesterol is important for ascertaining the health of fish. A needle-type biosensor system was developed for rapid and simple determination of glucose levels in fish blood. The sensor comprises a needle-type hollow container (18-gauge needle), immobilized enzyme membrane and optic fiber probe with ruthenium complex. The enzyme membrane was prepared from glucose oxidase, azide-unit pendant water-soluble photopolymer and an ultra-thin dialysis membrane. The optic fiber probe was inserted into the rolled enzyme membrane placed in the needle-type hollow container. The calibration curve was linear for glucose levels in fish plasma. One assay was completed within 3 min. A good reproducibility was observed for 60 times without exchange of the enzyme membrane. The sensor was inserted into the caudal vein of fish to measure blood glucose levels. The sensor responded immediately after insertion and glucose levels could be monitored. Good correlations were observed between values determined using the sensor and conventional methods in the range of 48-157 mg d1-1 (correlation coefficient, 0.9474).
近年来,病原性细菌感染的大规模爆发导致大量鱼类死亡,给养鱼场造成了巨大的经济损失。因此,养鱼场必须定期检查鱼类的健康状况,并尽快发现异常情况。一些研究表明,血糖水平与鱼类的应激水平密切相关,并代表呼吸或营养紊乱的状态。最近的研究还表明,血液胆固醇水平的降低是细菌感染抵抗力降低的一个有用指标。因此,血糖和胆固醇的测定对于确定鱼的健康是重要的。建立了一种快速、简便测定鱼血中葡萄糖含量的针式生物传感器系统。该传感器由针型空心容器(18号针)、固定化酶膜和钌配合物光纤探针组成。以葡萄糖氧化酶、含叠氮单元的水溶性光致聚合物和超薄透析膜为原料制备酶膜。将光纤探针插入放置在针型中空容器中的卷起的酶膜中。鱼血浆中葡萄糖水平的校准曲线呈线性。一次测定在3 min内完成,重复性好,可重复测定60次,无需更换酶膜。将传感器插入鱼的尾静脉以测量血糖水平。探头在插入后立即响应,可以监测葡萄糖水平。在48-157 mg d1-1(相关系数为0.9474)的范围内,使用传感器和传统方法测定的值之间观察到良好的相关性。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
「未来を拓くユビキタス・バイオセンシング」食としての魚の安全性をセンシングする(分筆,P247-259)
“无处不在的生物传感,开辟未来”感知鱼类食品的安全性(平装本,P247-259)
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:遠藤英明;(監修 三林浩二);遠藤英明(三林浩二編)
- 通讯作者:遠藤英明(三林浩二編)
A needle-type optical enzyme sensor system for determining glucose levels in fish blood
- DOI:10.1016/j.aca.2006.04.068
- 发表时间:2006-07-28
- 期刊:
- 影响因子:6.2
- 作者:Endo, Hideaki;Yonemori, Yuki;Mitsubayashi, Kohji
- 通讯作者:Mitsubayashi, Kohji
ユビキタス・バイオセンシング : 第4編第4章 : 食としての魚の安全性をセンシングする
无处不在的生物传感:第 4 卷第 4 章:感知鱼类食品的安全性
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:H.Endo;Y.Yonemori;K.Musiya;M.Maita;T.Shibuya;H.Ren;T.Hayashi;K.Mitsubayashi;遠藤英明(監修 三林浩二)
- 通讯作者:遠藤英明(監修 三林浩二)
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ENDO Hideaki其他文献
ENDO Hideaki的其他文献
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{{ truncateString('ENDO Hideaki', 18)}}的其他基金
Development of biosensor systems for fish health screening
鱼类健康筛查生物传感器系统的开发
- 批准号:
23380122 - 财政年份:2011
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of implantable wireless-biosensor for fish
鱼类植入式无线生物传感器的开发
- 批准号:
20380119 - 财政年份:2008
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Cause inquiry of root fracture in endodontic and periodontic fields-Analysis of the weak factors using three-dimensional photoelastic model tests-
牙髓、牙周领域牙根折裂原因探究-三维光弹模型试验薄弱因素分析-
- 批准号:
19592194 - 财政年份:2007
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The colonizing of periodontopathic bacteria and the transition of inflammation with dental calculus formation periodontal rat model.
牙周病细菌的定植和炎症的转变与牙结石形成牙周大鼠模型。
- 批准号:
14571976 - 财政年份:2002
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Rapid detection of bacterial cells using photon correlation spectroscope technique on HACCP
HACCP 中利用光子相关光谱技术快速检测细菌细胞
- 批准号:
13660201 - 财政年份:2001
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Relationships between inducing genes of nitric oxide synthase and apoptosis in the inflammatory gingival tissue.
一氧化氮合酶诱导基因与炎症牙龈组织凋亡的关系
- 批准号:
08672177 - 财政年份:1996
- 资助金额:
$ 1.73万 - 项目类别:
Grant-in-Aid for Scientific Research (C)