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Development of needle-type biosensor system for monitoring of fish health

开发用于监测鱼类健康的针型生物传感器系统

基本信息

批准号：
17580180
负责人：
ENDO Hideaki
金额：
$ 1.73万
依托单位：
Tokyo University of Marine Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Mass outbreaks of pathogenic bacterial infections have killed large quantities of fish in recent years, causing great financial damage to fish farms. Periodic checks of fish health and detection of abnormalities as quickly as possible are thus necessary at fish farms. Several studies have shown blood glucose levels closely correlate to the level of stress in fish and represent the state of respiratory or nutritional disturbance. Recent studies have also clarified that decreased levels of blood cholesterol represent a useful indicator of reduced resistance to bacterial infection. In this manner, determination of blood glucose and cholesterol is important for ascertaining the health of fish. A needle-type biosensor system was developed for rapid and simple determination of glucose levels in fish blood. The sensor comprises a needle-type hollow container (18-gauge needle), immobilized enzyme membrane and optic fiber probe with ruthenium complex. The enzyme membrane was prepared from glucose oxidase, azide-unit pendant water-soluble photopolymer and an ultra-thin dialysis membrane. The optic fiber probe was inserted into the rolled enzyme membrane placed in the needle-type hollow container. The calibration curve was linear for glucose levels in fish plasma. One assay was completed within 3 min. A good reproducibility was observed for 60 times without exchange of the enzyme membrane. The sensor was inserted into the caudal vein of fish to measure blood glucose levels. The sensor responded immediately after insertion and glucose levels could be monitored. Good correlations were observed between values determined using the sensor and conventional methods in the range of 48-157 mg d1-1 (correlation coefficient, 0.9474).

近年来，病原性细菌感染的大规模爆发导致大量鱼类死亡，给养鱼场造成了巨大的经济损失。因此，养鱼场必须定期检查鱼类的健康状况，并尽快发现异常情况。一些研究表明，血糖水平与鱼类的应激水平密切相关，并代表呼吸或营养紊乱的状态。最近的研究还表明，血液胆固醇水平的降低是细菌感染抵抗力降低的一个有用指标。因此，血糖和胆固醇的测定对于确定鱼的健康是重要的。建立了一种快速、简便测定鱼血中葡萄糖含量的针式生物传感器系统。该传感器由针型空心容器（18号针）、固定化酶膜和钌配合物光纤探针组成。以葡萄糖氧化酶、含叠氮单元的水溶性光致聚合物和超薄透析膜为原料制备酶膜。将光纤探针插入放置在针型中空容器中的卷起的酶膜中。鱼血浆中葡萄糖水平的校准曲线呈线性。一次测定在3 min内完成，重复性好，可重复测定60次，无需更换酶膜。将传感器插入鱼的尾静脉以测量血糖水平。探头在插入后立即响应，可以监测葡萄糖水平。在48-157 mg d1-1范围内，使用传感器和传统方法测定的值之间观察到良好的相关性（相关系数，0.9474）。