The colonizing of periodontopathic bacteria and the transition of inflammation with dental calculus formation periodontal rat model.

在牙结石形成后，给大鼠接种被认为是牙周病细菌的牙龈卟啉单胞菌（p.g.） 2周（每周3次）。接种前用磺胺甲恶唑/甲氧苄啶对特异性病原菌进行还原，清除口腔致病菌。经抗菌药物治疗后，接种P.g.。按Bringer等（1971）的处方给予大鼠牙石形成食物4周。将大鼠分为4组，1组为阴性对照（不接种细菌），其余3组换种。P.g.培养于血琼脂（以5.0μg/ml血红素、0.5μg/ml美那酮和5%去纤羊血为基础的哥伦比亚CNA）上。实验中，细菌生长在37℃的补血琼脂上，在10%二氧化碳、10%氢气和80%氮气的厌氧室内。用5%羧甲基纤维素在PBS溶液中接种活p.g.a悬液。将细菌直接接种于大鼠口腔。采用Bringer et al（1971）的方法估计牙石的粘连程度，评估牙槽骨缺损的吸收程度。虽然牙石的粘结力在下颌一侧粘附较多，但pg菌的沉降呈逐渐减少的趋势。在牙槽骨吸收度接种p.g.细菌的情况下，牙石的粘连度呈大趋势，与低无关。

Rats were inoculated 2 weeks (three times per week) with Porphyromonas gingivalis (P.g.) that is thought as the periodontopathic bacteia, after dental calculus had been formed. Before inoculation, the specific pathogen were reduced with sulfamethoxazole/trimethoprim and were eliminated oral pathogenic bacteria. After the medicament of anti-bacterial drugs, P.g. was inoculated. The dental calculus formation food by the prescriptions of Bringer et al (1971) was given to rats for 4 weeks. Rats were divided into 4 groups, 1 group was negative control (bacteria was not inoculated), 3 remaining groups were changed breed periods. P.g. was cultivated on supplemented blood agar (Colombia CNA based with 5.0μg/ml hemin, 0.5μg/ml menadione and 5% defibrinated sheep's blood). For experiments, bacteria were grown on supplemented blood agar at 37℃ in an anaerobic chamber under an atmosphere of 10% carbon dioxide, 10% hydrogen and 80% nitrogen. The rats were inoculated live P.g.suspension in 5% carboxymethyl-cellulose in PBS solutions. The bacteria were inoculated directly in the oral cavity of rats. It was estimated the adhesion of calculus with the method of Bringer et al(1971), and evaluated the absorption degree of an alveolar bone defects. Although the adhesion of the calculus is adhering a lot on the side of the lower jaw as a result, the settlement of the P.g.bacteria was in a decrease trend gradually. Also, the adhesion degree of the calculus was in a big trend, without being related to low, in the case that the absorption degree of the alveolar bone inoculated the P.g.bacteria.