Relationships between inducing genes of nitric oxide synthase and apoptosis in the inflammatory gingival tissue.

一氧化氮合酶诱导基因与炎症牙龈组织凋亡的关系

基本信息

批准号：
08672177
负责人：
ENDO Hideaki
金额：
$ 1.47万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Inducing Nitric Oxide Synthase in the Rat Inflammatory Gingival TissueThe distribution of 3 kinds of nitric oxide synthase ( oriented from brain, endothelial cell and macrophage ; NOS ) has been investigated in rat healthy and experimental inflamed gingiva. The samples were examined by FITC-conjugated antibody immuno-histochemically. In the part of FITC-positive area, the 3 kinds of NOS antibody were stained in 3 different width and concentration. The anti-macrophage NOS antibody area was the widest FITC-positive area. The other 2 NOS antibody staining area were observed in the localized severe inflammatory gingival area. The polymorphonuclear leukocytes (PMNs) have constitutive NOS and in the PMNs the stimulant does not induce NOS.In the experiment, 3 kinds of mouse-monoclonal NOS antibodies reacted in the inflammatory gingiva. It was suggested that the each 3 NOS antibody has a fragment matching to the reacted area of PMNs' NOS.Induction of apoptosis in HL-60 by periodonto-pathic bacteria and nitric oxidePolymorphonuclear leukocytes gather acute inflammatory gingivalarea and after the acute phase, lymphocytes gather the chronic inflamed gingiva. HL-60 (derived from acutepremyelotic leukemia) is original cell of PMNs and lymphocyte that are infection related cells. In this study, HL-60 and periodonto-pathic bacteria were incubated with and without NOR4 that has been auto-released nitric oxide. Apoptosis cells were estimated the divided shape of nucleic acid stained by fluorescent dye ( Hoechst 33342, acridine orange and ethidium bromide). The periodont-pathic bacteria were used Porphyromonas gingivalis W50, W83, ATCC 33277, Prevotella intermedia ATOC 25611, 26261. The results were as follows ; the apoptosis cells were detected all cases, differences among those bacteria were not detected. The presence of NOR4 accelerated apoptosis cells. The total average apoptosis cells were about 12% without stimulant in 3 days incubation.

已经研究了大鼠健康且实验性的炎症牙龈，研究了三种一氧化氮合酶（从脑，内皮细胞和巨噬细胞定向）的三种一氧化氮合酶（从脑，内皮细胞和巨噬细胞定向）的诱导一氧化氮合酶的分布。通过FITC偶联抗体免疫 - 归化检查样品。在FITC阳性区域的一部分中，将3种NOS抗体以3种不同的宽度和浓度染色。抗巨噬细胞NOS抗体面积是FITC阳性最大的区域。在局部的严重炎症牙龈区域中观察到其他2种NOS抗体染色区域。多形核白细胞（PMN）具有构成性的NOS，在PMN中，刺激剂不会诱导NOS。在实验中，在炎症性牙龈中反应3种小鼠单球体NOS NOS抗体。 It was suggested that the each 3 NOS antibody has a fragment matching to the reacted area of PMNs' NOS.Induction of apoptosis in HL-60 by periodonto-pathic bacteria and nitric oxidePolymorphonuclear leukocytes gather acute inflammatory gingivalarea and after the acute phase, lymphocytes gather the chronic inflamed gingiva. HL-60（源自阿卡特省核酸白血病）是与感染相关细胞的PMN和淋巴细胞的原始细胞。在这项研究中，将HL-60和牙周病细菌与已自动释放的一氧化氮的NOR4一起孵育。细胞凋亡细胞被估算为荧光染料染色的核酸的划分形状（Hoechst 33342，Acridine Orange和Bromide）。使用牙周病毒细菌W50，W83，ATCC 33277，Prevotella Intermedia Intermedia atoc 25611，26261。结果如下：如下；检测到所有病例的凋亡细胞，未检测到这些细菌之间的差异。 NOR4的存在加速了凋亡细胞。总平均凋亡细胞约为12％，没有刺激性在3天的孵育中。