Development of biosensor systems for fish health screening

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We developed novel biosensor systems for fish health screening. Various implantable wireless-biosensors to monitor fish health indicators, such as glucose, cholesterol, and lactic acid, were prepared. These sensor systems allow us to monitor the health indicators in fish under free-swimming conditions. We also developed a label-free immunosensor system for detecting the oocyte maturation-inducing hormone (DHP). The mechanism of the sensor system is based on differences in electrochemical activity changed by an immunoreaction in the absence and presence of DHP. One sample can be used to measure DHP levels within as little as 15 min. Moreover, we developed a novel method for detecting Flavobacterium psychrophilum using high-gradient immunomagnetic separation with polymerase chain reaction, which allows for ultra-highly sensitive and specific detection of F. psychrophilum. These newly developed biosensor systems allow for rapid and convenient monitoring of fish health indicators.