Development of biosensor systems for fish health screening

鱼类健康筛查生物传感器系统的开发

• 批准号: 23380122
• 负责人: ENDO Hideaki
• 金额: $ 10.32万
• 依托单位: Tokyo University of Marine Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380122/
• 关键词: バイオセンサ; 魚類; 健康診断; モニタリング; リアルタイム; カーボンナノチューブ; 生体適合性; in vivo; グルコース; 乳酸; 血糖値; 卵成熟; 計測

We developed novel biosensor systems for fish health screening. Various implantable wireless-biosensors to monitor fish health indicators, such as glucose, cholesterol, and lactic acid, were prepared. These sensor systems allow us to monitor the health indicators in fish under free-swimming conditions. We also developed a label-free immunosensor system for detecting the oocyte maturation-inducing hormone (DHP). The mechanism of the sensor system is based on differences in electrochemical activity changed by an immunoreaction in the absence and presence of DHP. One sample can be used to measure DHP levels within as little as 15 min. Moreover, we developed a novel method for detecting Flavobacterium psychrophilum using high-gradient immunomagnetic separation with polymerase chain reaction, which allows for ultra-highly sensitive and specific detection of F. psychrophilum. These newly developed biosensor systems allow for rapid and convenient monitoring of fish health indicators.

我们开发了用于鱼类健康筛查的新型生物传感器系统。制备了各种可植入的无线生物传感器来监测鱼类健康指标，例如葡萄糖，胆固醇和乳酸。这些传感器系统使我们能够在自由锻炼条件下监视鱼类的健康指标。我们还开发了一种无标记的免疫传感器系统，用于检测卵母细胞成熟的激素（DHP）。传感器系统的机理是基于在不存在和存在DHP的情况下通过免疫反应而改变的电化学活性的差异。一个样品可用于在15分钟内测量DHP水平。此外，我们开发了一种新的方法，该方法使用高梯度免疫磁性分离，通过聚合酶链反应检测精神杆菌，这允许对F. spectrophillum进行超高敏感和特异性检测。这些新开发的生物传感器系统可快速快速地监测鱼类健康指标。

项目成果

Carbon nanotube enhanced mediator-type biosensor for real-time monitoring of glucose concentrations in fish

碳纳米管增强介体型生物传感器实时监测鱼体内葡萄糖浓度

• 发表时间: 2012
• 期刊: Anal. Bioanal. Chem
• 作者: M. Takase;Y. Yoneyama;M. Murata;K. Hibi;H. Ren;H. Endo
• 通讯作者: H. Endo

Development of mediator-type biosensor to wirelessly monitor whole cholesterol concentration in fish

开发介质型生物传感器来无线监测鱼中的总胆固醇浓度

• DOI: 10.1007/s10695-013-9851-1
• 发表时间: 2014
• 期刊: Fish Physiol. Biochem
• 作者: M. Takase;M. Murata;K. Hibi;R. Huifeng;H. Endo
• 通讯作者: H. Endo

Label-free immunosensor using carbon nanotube for amperometric determination of oocyte maturation-inducing hormone in fish

使用碳纳米管的无标记免疫传感器安培测定鱼类卵母细胞成熟诱导激素

• 发表时间: 2011
• 作者: M. Hirai;T. Muramatsu;H. Ohnuki;G. Yoshizaki;K. Hibi;H. Ren;H. Endo
• 通讯作者: H. Endo

Ultra highly sensitive method for detecting Flavobacterium psychrophilum using high-gradient immunomagnetic separation with polymerase chain reaction

聚合酶链反应高梯度免疫磁分离检测嗜冷黄杆菌的超灵敏方法

• 发表时间: 2012
• 期刊: Aquaculture Res
• 作者: U. Ryumae;K. Hibi;Y. Yoshiura;H. Ren;H. Endo
• 通讯作者: H. Endo

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