Rapid detection of bacterial cells using photon correlation spectroscope technique on HACCP

HACCP 中利用光子相关光谱技术快速检测细菌细胞

基本信息

批准号：
13660201
负责人：
ENDO Hideaki
金额：
$ 2.5万
依托单位：
Tokyo University of Marine Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

We developed new system for rapid detection of bacteria cells using photon correlation spectroscope (PCS) technique.1.Removal of bacterial cells by ultrasonic vibrationTo remove bacterial cells from the surface of seafood products (Surimi-based products, fish), ultrasonic energy was used. Almost of all cells can be removed from seafood in 5 min with ultrasonic treatment. Flow cytometry (FCM) technique applied to the determination of total number of microbial cells grown on seafood products. Cell numbers determined by FCM well paralleled that measured by traditional colony counting method in the range of 105-109 cells/g. One FCM assay could be completed within 60 sec and the total assay time including the preparation of microbial cells was within 30 min.2.Rapid detection of Salmonella cells by PCS techniqueRapid detection system for nitrifying bacteria was developed using PCS technique. The method is based on that Salmonella cells reacted with anti-Salmonella antibody were able to analyze by PCS technique. When Salmonella cells were reacted with the antibody beads, the size distribution was shifted to larger range. In contrast, in the reference bacterial cells (Escherichia coli), the size distribution shows little change. The phenomenon was assumed that since the antibody reacted with Salmonella cells selectively, they were aggregated with bacterial cells, and the distribution size increased. Thus, it is possible to discriminate between aimed nitrifying bacteria and other spices of bacterial cells using PCS technique. One assay could be completed in 10 min, and the total PCS assay time including sample preparation was within 60 min.

我们开发了使用光子相关光谱仪（PCS）技术快速检测细菌细胞的新系统。1。通过超声振动驱动细菌细胞，以从海鲜产物（基于Surimi的产物，FISH）的表面去除细菌细胞，使用超声波能量。超声处理几乎可以在5分钟内从海鲜中除去所有细胞中的几乎所有细胞。流式细胞仪（FCM）技术应用于测定海鲜产品上生长的微生物细胞总数。通过FCM确定的细胞数很好地平行于传统菌落计数方法在105-109细胞/g的范围内测量。可以在60秒内完成一个FCM测定法，其中包括微生物细胞的制备在内的总测定时间在30分钟内。2。使用PCS技术通过PCS技术进行了PCS Techniquerapid检测系统，用于使用PCS技术开发用于硝化细菌。该方法基于的是，与抗盐酸抗体反应的沙门氏菌细胞能够通过PCS技术进行分析。当沙门氏菌细胞与抗体珠反应时，尺寸分布转移到较大的范围。相反，在参考细菌细胞（大肠杆菌）中，大小分布几乎没有变化。假定该现象是，由于抗体与沙门氏菌细胞有选择地反应，因此它们与细菌细胞聚集，分布尺寸增加。因此，可以使用PCS技术区分瞄准的硝化细菌和细菌细胞的其他香料。一个测定方法可以在10分钟内完成，并且包括样品制备在内的总PC测定时间在60分钟内。