IDENTIFICATION AND RECOVERY OF MOLECULES IN BRAIN

脑分子的识别和回收

• 批准号: 17580260
• 负责人: KATO Keiko
• 金额: $ 2.24万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580260/
• 关键词: neural plasticity; epilepsy; neural circuit; microinjection; model mice

I have focused on kindling mice, which is an epileptic model and induces abnormal neural plasticity with changes in neural circuits. The purpose in this grant is to investigate a flow of information at the molecular level occurring in a series of neural nuclei during stimulation of kindling and to evaluate molecular processes in acquirement of neural plasticity.In 2005, I have developed to prepare kindling mice containing microinjection cannula, in which an anode electrode and the cannula were implanted into the basolateral amygdaloid complex and the hippocampus, respectively. The model mice are a system causing both of the kindling stimulation and administration of a drug. At the same time, I have identified a molecular candidate A up-regulated tremendously during kindling epileptogenesis.In 2006, I have injected the molecular candidate A into the present kindling mice, resulting in accelerating stages of kindling-epileptogenesis being dependent on the injection of the molecular candidate A. The result suggests that molecular candidate A involves in inducing epilepsy. Now, I prepare a journal article based on a series of data, which has been supported by this grant.

我的重点是点燃小鼠，这是一种癫痫模型，通过改变神经回路来诱导异常的神经可塑性。这项资助的目的是研究点燃刺激时一系列神经核团在分子水平上的信息流，并评估获得神经可塑性的分子过程。2005年，我开发了一种含有微量注射套管的点燃小鼠的制备方法，在该导管中，阳极电极和套管分别植入基底外侧杏仁复合体和海马区。模型小鼠是一个既能引起点燃刺激又能给药的系统。同时，我还发现了一个在癫痫点燃过程中表达显著上调的候选分子A。2006年，我在目前的点燃小鼠体内注射了候选分子A，导致点燃-癫痫发生的加速阶段依赖于候选分子A的注射。现在，我在一系列数据的基础上准备了一篇期刊文章，这篇文章得到了这笔拨款的支持。

项目成果

Distributions of glucuronyltransferases, GlcAT-P and GlcAT-S, and their target substrate, the HNK-1 carbohydrate epitope in the adult mouse brain with or without a targeted deletion of the GlcAT-P gene

• DOI: 10.1016/j.brainres.2007.05.012
• 发表时间: 2007-11-07
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Inoue, Mitsuhiro;Kato, Keiko;Oka, Shogo
• 通讯作者: Oka, Shogo

Glycobiological approach to understanding neural plasticity.

理解神经可塑性的糖生物学方法。

• 发表时间: 2007
• 期刊: Trends in glycoscience and glycotechnology (TIGG) 19-106
• 作者: Morishita;Y.;Matsuzaki;T.;Ikeda;M.;et al.;Bah MM;池田正浩;Kato K
• 通讯作者: Kato K

Glycobiological approaches for understanding neural plasticity

理解神经可塑性的糖生物学方法

• 发表时间: 2007
• 期刊: Trends in glycoscience and glycotechnology (In press)
• 作者: Masashi Takahashi;Miki Sakatani;Shotaro Nishimura;Kato K
• 通讯作者: Kato K

小脳グリア細胞の分化機構-糖鎖生物学の視点から-

小脑胶质细胞的分化机制——从糖生物学的角度——

• 发表时间: 2005
• 期刊: CLINICAL NEUROSCIENCE(臨床神経科学) 23
• 作者: 加藤啓子;平林義雄
• 通讯作者: 平林義雄