IDENTIFICATION AND RECOVERY OF MOLECULES IN BRAIN

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I have focused on kindling mice, which is an epileptic model and induces abnormal neural plasticity with changes in neural circuits. The purpose in this grant is to investigate a flow of information at the molecular level occurring in a series of neural nuclei during stimulation of kindling and to evaluate molecular processes in acquirement of neural plasticity.In 2005, I have developed to prepare kindling mice containing microinjection cannula, in which an anode electrode and the cannula were implanted into the basolateral amygdaloid complex and the hippocampus, respectively. The model mice are a system causing both of the kindling stimulation and administration of a drug. At the same time, I have identified a molecular candidate A up-regulated tremendously during kindling epileptogenesis.In 2006, I have injected the molecular candidate A into the present kindling mice, resulting in accelerating stages of kindling-epileptogenesis being dependent on the injection of the molecular candidate A. The result suggests that molecular candidate A involves in inducing epilepsy. Now, I prepare a journal article based on a series of data, which has been supported by this grant.