Assessment of phylogeny, virulence genes and O serogroup profiles in Escherichia coli from uncomplicated and complicated cystitis and from asymptomatic bacteriuria

对单纯性和复杂性膀胱炎以及无症状菌尿大肠杆菌中的系统发育、毒力基因和 O 血清群谱进行评估

• 批准号: 17591677
• 负责人: YAMAMOTO Shingo
• 金额: $ 2.18万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591677/
• 关键词: UPEC (uropathogenic E. coli); complicated cystitis; ASB (asymptomatic bacteriuria); phylogenetic group; O serogroup; virulence factor gene; 病原遺伝子; 単純性膀胱炎

Purpose: There have been only a few studies to clarify the genetic characteristics of microorganisms isolated from complicated urinary tract infection (UTI) and asymptomatic bacteriuria (ASB). In the present study, we determined prevalence of phylogenetic groups, O serotypes, and virulence factor (VF) genes, in E. coli isolates from umcomplicated cystitis (UC), complicated cystitis (CC), and complicated asymptomatic bacteriuria (CASB).Materials and Methods: Three hundred and forty seven E.coli isolates (204 from UC, 66 from CC, and 77 from CASB) were collected. Polymerase chain reaction (PCR) assays were conducted for phylogenetic analysis and detection of 18 VF genes (aer, afa, cnfl, cvaC, ETTT, fimH, fyuA, hly, ibeA, iha, iroN, kpsMT, ompT, PAI, pap, sfa/foc, traT and usp). Further, the isolates were subjected to O serotyping.Results: Phylogenetic group B2 was shown to be predominant in all categories (UC: 71 %, CC: 73%, and CSAB: 61 %). There was no significant difference in distribution of common uropathogenic O serotypes among the three categories. (UC: 59 %, CC: 50%, and CSAB: 46 %). Of 18 virulence genes examined, iroN, pap, ompT, and PAI were more frequently discovered in UC than in CC or CSAB, whereas the prevalence of other 14 genes were similar among the three categories.Conclusions: E. coli isolates from CC and CASB shared almost similar characters and properties with UC isolates in terms of phylogenetic, O serotypes, and VFs, although it is likely that more number of VFs are requird in causing uncomplicated or symptomatic UTI than complicated or asymptomatic infections. The development of symptomatic infection may be more closely associated with variation of the host environment rather than with VFs expressed by microorganism.

目的：只有少数研究阐明了从复杂性尿路感染（UTI）和无症状菌尿（ASB）中分离的微生物的遗传特征。在本研究中，我们确定了流行的系统发育群，O血清型，毒力因子（VF）基因，在大肠杆菌。材料与方法：收集347株大肠杆菌，其中单纯性膀胱炎（UC）204株，复杂性膀胱炎（CC）66株，复杂性无症状菌尿（CASB）77株。采用聚合酶链反应（PCR）方法对18个VF基因（aer、afa、cnfl、cvaC、ETTT、fimH、fyuA、hly、ibeA、iha、iroN、kpsMT、ompT、派、pap、sfa/foc、traT和usp）进行系统发育分析和检测。结果：B2群占优势（UC：71%，CC：73%，CSAB：61%）。3类人群中常见的O型分布无显著性差异。(UC：59%，CC：50%，和CSAB：46%）。在检测的18个毒力基因中，iroN、pap、ompT和派在UC中的检出率高于CC和CSAB，而其他14个基因的检出率在3种类型中相似。CC和CASB分离株与UC分离株在系统发育、O血清型和VF方面具有几乎相似的特征和性质，尽管与复杂或无症状的感染相比，引起无并发症或有症状的UTI可能需要更多的VF。症状性感染的发展可能与宿主环境的变化更密切相关，而不是与微生物表达的VF。

项目成果

Escherichia coli from uncomplicated and complicated cystitis and from asymptomatic bacteriuria possess similar phylogeny, virulence genes and O serogroup profiles

来自单纯性和复杂性膀胱炎以及无症状菌尿的大肠杆菌具有相似的系统发育、毒力基因和 O 血清群谱

• 发表时间: 2006
• 期刊: Journal of Clinical Microbiology 44・12
• 作者: Takahashi A.;Kanamaru S.;Kurazono H.;Kunishima Y.;Tsukamoto T.;Ogawa O.;Yamamoto S.
• 通讯作者: Yamamoto S.

Escherichia coli from uncomplicated and complicated cystitis and from asymptomatic bacteriuria possess similar phylogeny, virulence genes and O serogroup profiles.

来自单纯性和复杂性膀胱炎以及无症状菌尿的大肠杆菌具有相似的系统发育、毒力基因和 O 血清群谱。

• 发表时间: 2006
• 期刊: Journal of Clinical Microbiology 44, 12
• 作者: Takahashi A.;Kanamaru S.;Kurazono H.;Kunishima Y.;Tsukamoto T.;Ogawa O.;Yamamoto S.
• 通讯作者: Yamamoto S.

Escherichia coli from uncomplicated and complicated cystitis and froasymptomatic bacteriuria possess similar phylogeny, virulence genes an O serogroup profiles.

来自单纯性和复杂性膀胱炎和无症状菌尿的大肠杆菌具有相似的系统发育、毒力基因和 O 血清群特征。

• 发表时间: 2006
• 期刊: J Clin Microbiol 44・12
• 作者: Takahashi A;Kanamaru S;Kurazono H;Kunishima Y;Tsukamoto T;Ogawa O;Yamamoto S
• 通讯作者: Yamamoto S