Induction of fluoroquinolone-resistant uropathogenic Escherichia coli using animal models

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Five fluoroquinolone-resistant (4- 32μ g/ml) Escherichia coli strains were developed by culture of 89 fluoroquinolone-susceptible isolates on agars including low to high concentration of levofloxacin. The original strains of these 5 resistant strains had one or two point-mutations in quinolone resistance-determining regions (QRDR). Further,the 5 resitant strains showed high RNA expression of marA, an efflux pump for antibiotics, determined by real time PCR. These results showed that a few ponint-mutations in QRDR and high expression of marA would be required as the first step for the acquisition of fluoroquinolone-resistance, indicating that inhibition of the efflux pump for antibiotics could be a good candidate for the strategy to prevent the induction of fluoroquinolone-resistance in treatment of urinary tract infections caused by uropathogenic E. coli.