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Development of novel bioabsorbable synthetic grafts for ureteral regeneration

开发用于输尿管再生的新型生物可吸收合成移植物

基本信息

批准号：
18591750
负责人：
YAMAMOTO Shingo
金额：
$ 2.42万
依托单位：
Hyogo College of Medicine (2007)Kyoto University (2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591750/
关键词：
Ureteral regeneration PLA-CL mesh Collagen coating bFGF sustained-release geratin sheet

项目摘要

We have previously reported that the deficient urethras had been successfully regenerated by surgically replacing with the bioabsorbable synthetic tube grafts composed of poly-(lactide/ -caprolactone) copolymer and collagen (Kanatani, et. Al.: Tissue Engineering 13: 2933,2007). Based on these techniques and results, the tube grafts composed of the identical materials were prepared for urethral regeneration. However, unfortunately, the replacement of the rabbit ureter was technically difficult due to the fine diameters of those.Next, we hypothesized that the urinary tract can be repaired by simply wrapping from outside with appropriate materials. At this point, we focused on the behavior of smooth muscle and extracellular matrix. To simplify our experimental design, the rat bladder wall was covered by a gelatin sheet releasing bFGF. As a result, bFGF promoted the bladder smooth muscle proliferation and matrix remodeling, which reinforced the overall bladder contractility (Imamura et.al.:Imamura, et. Al.: American Journal of Physiology. Renal Physiology 293:F1007,2007).Finally, the rat ureter was wrapped with a bFGF sustained-release geratin sheet. Two weeks later urethral wall hypertrophy associated with a capillary angiogenesis was observed. There will be a need to elucidate whether this phenomenon contributes to functional regeneration of the ureter.

我们以前曾报道，通过手术替换由聚丙交酯/己内酯共聚物和胶原(Kanatani等)组成的可生物吸收的合成管移植物，成功地再生了缺失的尿路。美国：组织工程13：2933,2007)。在这些技术和结果的基础上，用相同材料制成的管状移植物用于尿路再生。然而，不幸的是，由于兔输尿管的直径很小，更换输尿管在技术上是困难的。接下来，我们假设只需从外面用合适的材料包裹就可以修复尿路。在这一点上，我们重点研究了平滑肌和细胞外基质的行为。为了简化实验设计，在大鼠膀胱壁上覆盖了一层释放碱性成纤维细胞生长因子的明胶薄膜。因此，bFGF促进了膀胱平滑肌的增殖和基质的重塑，从而增强了整体膀胱的收缩能力(Imamura等人：Imamura等人)。美国生理学杂志。肾生理学293：F1007,2007)。最后，用碱性成纤维细胞生长因子缓释Geratin薄膜包裹大鼠输尿管。两周后观察到尿道壁肥大伴毛细血管生成。有必要阐明这一现象是否有助于输尿管的功能性再生。