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Clarification of mechanisms for delayed re-epithelialization of chronic wounds -to establish treatment based on wound healing theory-

澄清慢性伤口上皮化延迟的机制-建立基于伤口愈合理论的治疗-

基本信息

批准号：
17591882
负责人：
KUBO Miyoko
金额：
$ 2.24万
依托单位：
Kawasaki Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591882/
关键词：
chronic wounds pressure ulcers re-epithelialization burns α5β1 fibronection laminin-1 褥瘡 αvβ6 ラミニン

项目摘要

To clarify the mechanisms of delayed re-epithelialization in chronic wounds, we examined the changes in α5β1 expression in the migrating epidermis, fibronectin (FN) distribution under the migrating epidermis, laminin-1expression at the dermal-epidermal junction and histological fmdings for pressure ulcers compared with those for bums (acute wounds, control). Four μm of paraffin sections in a serial of 22 samples with 20 pressure ulcers and 24 samples with 24 burns were stained for α5β1, FN and laminin-1 by a peroxidase immunohistochemical method. The sections were also stained with hematoxylin and eosin (HE). In these immunostained and HE stained specimens, we measured the distance of positive expression of α5β1, increased distribution of FN, negative or decreased expression of laminin-1, histological epidermal elongation (portion showing parakeratosis) and epidermal thickening using IPLab. We then analyzed the correlation of those findings within each group of burns or pressure ulcers … More . Next we sorted the differences in the findings between the two groups. With burn specimens, there was a statistically significant correlation between positive α5β1 and increased fibronectin, positive α5β1 and negative laminin-1, and increased fibronectin and negative laminin-1. In addition, epidermal elongation significantly correlated with positive α5β1, increased fibronectin, and negative laminin-1. With pressure ulcer specimens, a significant correlation was shown between positive α5β1 and increased FN, positive α5β1 and negative laminin-1, increased FN and epidermal elongation, and epidermal elongation and epidermal thickening. The pressure ulcer group showed a significant decrease in the distance of positive α5β1, increased FN, negative laminin-1 compared with the burn group. Increase in epidermal thickening was also significant. These data demonstrated that α5β1 and FN were the significant contributing factors in epidermal migration, and the decrease in α5β1 expression in the migrating epidemis and FN distribution under the migrating epidermis were considered to be mechanisms of delayed re-epithelialization with pressure ulcers. Less

为探讨慢性创面延迟上皮化的机制，我们观察了慢性创面移行表皮中α5β1的表达、移行表皮下纤维连接蛋白(FN)的分布、真皮-表皮交界处的层粘连蛋白-1(LN-1)表达的变化以及压疮与烧伤(急性创面，对照)的组织学基础。用过氧化物酶免疫组织化学方法对22例(20个压疮)和24例(24个烧伤)石蜡切片中的4个μm进行α-5β-1、FN和LN-1染色。切片行苏木精-伊红(HE)染色。免疫组织化学染色和HE染色观察α5β1阳性表达的距离、FN分布增多、层粘连蛋白1表达阴性或减少、组织学上皮伸长(部分角化不全)和表皮增厚。然后，我们分析了每组烧伤或压疮…中这些发现的相关性。更多。接下来，我们整理了两组研究结果的不同之处。烧伤组织中α-5-β-1阳性与纤维连接蛋白升高、α-5-β-1阳性与层粘连蛋白-1阴性、纤维连接蛋白与层粘连蛋白-1阴性呈显著正相关。此外，表皮伸长与α-5β-1阳性、纤维连接蛋白增多和层粘连蛋白-1阴性显著相关。压疮组织中α-5-β-1阳性与FN增加、α-5-β-1阳性与层粘连蛋白-1阴性、FN与表皮伸长增加、表皮伸长与表皮增厚呈显著正相关。压疮组与烧伤组比较，α-5、β-1阳性表达的距离缩短，FN增多，LN-1阴性。表皮增厚也显著增加。以上结果表明，α-5-β-1和FN是造成压疮表皮迁移的重要因素，迁移流行病中α-5-β-1表达的减少和迁移表皮下FN的分布被认为是压疮延迟上皮化的机制。较少

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Transduction of β 3 integrin subunit cDNA confers on human keratinocytes the ability to adhere to gelatin.

β 3 整合素亚基 cDNA 的转导赋予人角质形成细胞粘附明胶的能力。

DOI：
发表时间：
2007
期刊：
Arch Dermatol Res 299・1
影响因子：
0
作者：
Miyoko Kubo
通讯作者：
Miyoko Kubo

Ying Zhao and Takahiko Moriguchi : Transduction of β3 Integrin Subunit cDNA Confers on Human Keratinocytes the Ability to Adhere to Gelatin.

Ying Zhao 和 Takahiko Moriguchi：β3 整合素亚基 cDNA 的转导赋予人角质形成细胞粘附明胶的能力。

DOI：
发表时间：
2007
期刊：
Arch Dermatol Res 299-1
影响因子：
0
作者：
Miyoko Kubo;Richard A.F.Clark;Anne B.Katz;Lorne B.Taichman;Zaishun Jin
通讯作者：
Zaishun Jin

Transduction of β3 integrin subunit cDNA confers on human keratinocytes the ability to adhere to gelatin.

β3 整合素亚基 cDNA 的转导赋予人角质形成细胞粘附明胶的能力。

DOI：
发表时间：
2007
期刊：
Arch Dermatol Res 299・1
影响因子：
0
作者：
Miyoko Kubo;Richard A.F.Clark;Anne B.Katz;Lorne B.Taichman;Zaishun Jin;Miyoko Kubo
通讯作者：
Miyoko Kubo

ケラチノサイトの細胞増殖に与えるbFGFの効果について

bFGF 对角质形成细胞增殖的影响