Verification for the effectiveness of β3 integrin-recombinant cultured epithelium by animal experiments

我们通过动物实验验证了重组β3整合素培养上皮的有效性。用逆转录病毒介导的方法转染人角细胞β3整合素亚基cDNA。细胞分选富集β3阳性细胞（89%阳性），在纤维蛋白凝胶上培养5 d制成培养上皮。将培养的上皮细胞移植到裸鼠背部，用丝裂霉素C局部处理全层创面，建立创面愈合迟缓模型。由β-半乳糖苷酶介导的角质形成细胞组成的培养上皮作为对照。在3个时间点（1、2、3周）取标本进行组织学检查。由β3重组角质形成细胞组成的培养上皮在各时间点均有轻微至中等程度的摄取率增加。需要进一步的实验来确定培养的上皮在体内的有效性。

We verified the effectiveness ofβ3 integrin-recombinant cultured epithelium by animal experiments. Human kerainocytes were transduced withβ3 integrin subunit cDNA by a retrovirus-mediated transduction method.β3 positive cells were enriched by cell sorting (89% positive), and were cultured on fibrin gels for 5 days to make the cultured epithelium.The cultured epithelia were transplanted to the backs of nude mice on which full-thickness wounds were topically treated with Mitomycin C to make a retarded wound healing model. Cultured epithelia made ofβ-galactosidase cDNA-transduced keratinocytes were used as a control. Histological examination was done with the samples taken at three time points (1, 2, 3 weeks).Cultured epithelia made ofβ3-recombinant keratinocytes increased its take-rate in a slight to moderate degree at all time points.Further experiments should be done to determine the effectiveness of the cultured epithelium in vivo.