Verification for the effectiveness of β3 integrin-recombinant cultured epithelium by animal experiments

通过动物实验验证了β-3整合素重组培养上皮细胞的有效性。采用逆转录病毒介导的转导方法将β-3整合素亚单位基因导入人核基质细胞，经细胞分选获得β-3阳性细胞(阳性率为89%)，在纤维蛋白凝胶上培养5d后，将培养的上皮细胞移植到裸鼠背部，用丝裂霉素C局部处理全层创面，制成创面延迟愈合模型。以转导β-半乳糖苷酶基因的角质形成细胞为对照。分别于术后1、2、3周取材进行组织学检查，β-3重组角质形成细胞培养的上皮细胞在各时间点的取出率均有不同程度的增加，有待于进一步的体内实验研究。

We verified the effectiveness ofβ3 integrin-recombinant cultured epithelium by animal experiments. Human kerainocytes were transduced withβ3 integrin subunit cDNA by a retrovirus-mediated transduction method.β3 positive cells were enriched by cell sorting (89% positive), and were cultured on fibrin gels for 5 days to make the cultured epithelium.The cultured epithelia were transplanted to the backs of nude mice on which full-thickness wounds were topically treated with Mitomycin C to make a retarded wound healing model. Cultured epithelia made ofβ-galactosidase cDNA-transduced keratinocytes were used as a control. Histological examination was done with the samples taken at three time points (1, 2, 3 weeks).Cultured epithelia made ofβ3-recombinant keratinocytes increased its take-rate in a slight to moderate degree at all time points.Further experiments should be done to determine the effectiveness of the cultured epithelium in vivo.