Identification of the erythrocyte receptor for Plasmodium yoelii RhopH complex

约氏疟原虫 RhopH 复合物红细胞受体的鉴定

• 批准号: 16017272
• 负责人: TORII Motomi
• 金额: $ 9.6万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16017272/
• 关键词: infectious disease; malaria; Plasmodium; マラリア原虫; 赤血球結合蛋白質; レセプター; GPIアンカー

Parasitophorous vacuole formation is a critical step for the successful invasion of host erythrocytes by malaria parasite. Rhoptry proteins are believed to have essential roles in vacuole formation, although their biological roles are poorly understood. To understand the molecular interactions between parasite rhoptry proteins and the erythrocyte during invasion, we have characterized the binding specificity of the high molecular mass rhoptry protein (RhopH) complex to erythrocytes using the rodent malaria parasite, Plasmodium yoelii and proposed that the major erythrocyte receptor for PyRhopH complex is a protein attached to the erythrocyte surface via GPI-anchor.To further characterize and identify the unknown receptor protein, we employed forward genetic analysis using consomic mouse strains derived from C57BL/6J (B6) and MSM/Ms strains based on the difference in the PyRhopH complex binding to the erythrocytes from these mice (erythrocytes from B6 exhibited higher binding than MSM/Ms). These consomic strains have B6 genetic background and a particular chromosome or chromosomal region is replaced with the one from MSM/Ms. A panel of consomic strains available were examined and the binding among consomic strains were found to be not clearly differentiate to B6 type or MSM/Ms type, indicating that multiple factors were involved in the observed binding. However B6-11^<MSM/Ms>, which harbored chromosome 11 derived from MSM/Ms, showed a prominent low binding, even lower than MSM/Ms, thus we considered that the receptor protein potentially located on chromosome 11. Based on this assumption, we are in a process to narrow the candidate region on the chromosome 11 by further linkage analysis between B6 and B6-11^<MSM/Ms>. The GPI-anchored protein on the candidate region would be characterized as a prime candidate for the PyRhopH complex receptor.

寄生虫形成是成功入侵疟疾寄生虫宿主红细胞的关键步骤。据信，罗普特蛋白在液泡形成中具有重要作用，尽管它们的生物学作用知之甚少。为了了解寄生虫rhoptry蛋白与入侵期间寄生虫之间的分子相互作用，我们表征了使用啮齿动物疟原虫寄生虫，质子质量YOELII的高分子质量综合蛋白（Rhhoph）复合物与红细胞与红细胞的结合特异性，该蛋白酶与主要的互联网相关的IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS的肌料置于侵蚀薄片的复合物。通过GPI锚固的表面为了进一步表征和识别未知的受体蛋白，我们使用源自C57BL/6J（B6）和MSM/MS菌株的综合小鼠菌株基于pyRhoph复合物的差异与这些小鼠（Erythrocytiped by MSM MSM的erythocytes comploy）的伴侣菌株和MSM/MS菌株。这些综合菌株具有B6遗传背景，并且特定的染色体或染色体区域被MSM/MS的染色体区域取代。检查了一系列可用的综合菌株，并发现合成菌株之间的结合并未明显与B6型或MSM/MS类型区分开，这表明观察到的结合中涉及多个因素。 However B6-11^<MSM/Ms>, which harbored chromosome 11 derived from MSM/Ms, showed a prominent low binding, even lower than MSM/Ms, thus we considered that the receptor protein potentially located on chromosome 11. Based on this assumption, we are in a process to narrow the candidate region on the chromosome 11 by further linkage analysis between B6 and B6-11^<MSM/Ms>.候选区域的GPI锚定蛋白将被描述为pyRhoph复合物受体的主要候选者。

项目成果

Potent immunogenicity of DNA vaccines encoding Plasmodium vivax transmission-blocking vaccine candidates Pvs25 and Pvs28-evaluation of

编码间日疟原虫传播阻断候选疫苗 Pvs25 和 Pvs28 的 DNA 疫苗的有效免疫原性-评估

• 发表时间: 2004
• 期刊: Vaccine 22・23-24
• 作者: Kaneko 0;Yim-Lim BYS;Iriko H;Ling IT;Otsuki H;Grainger M;Tsuboi T;Adams JH;Mattei D;Holder AA;Torii M.;Kaneko O et al.;Winter G et al.;Kongkasuriyachai D et al.
• 通讯作者: Kongkasuriyachai D et al.

Apical expression of three RhopHl/Clag proteins as components of the Plasmodium falcioarum RhopH complex.

作为镰状疟原虫 RhopH 复合物成分的三种 RhopH1/Clag 蛋白的顶端表达。

• 发表时间: 2005
• 期刊: Mol Biochem Parasitol 143(1)
• 作者: Kaneko 0;Yim-Lim BYS;Iriko H;Ling IT;Otsuki H;Grainger M;Tsuboi T;Adams JH;Mattei D;Holder AA;Torii M.
• 通讯作者: Torii M.

2-Cys peroxiredoxin TPx-1 is involved in gametocyte development in Plasmodium berghei

• DOI: 10.1016/j.molbiopara.2006.02.018
• 发表时间: 2006-07-01
• 期刊: MOLECULAR AND BIOCHEMICAL PARASITOLOGY
• 影响因子: 1.5
• 作者: Yano, Kazuhiko;Komaki-Yasuda, Kanako;Kawazu, Shin-ichiro
• 通讯作者: Kawazu, Shin-ichiro

Nasal immunization with a malaria transmission-blocking vaccine candidate Pfs25 induces complete protective immunity in mice against field-isolated Plasmodium falciparum

使用阻断疟疾传播的候选疫苗 Pfs25 进行鼻免疫可诱导小鼠针对现场隔离的恶性疟原虫产生完全保护性免疫力

• 发表时间: 2005
• 期刊: Infection and Immunity 73
• 作者: Arakawa T;et. al.
• 通讯作者: et. al.

Apical expression of three RhopH1/Clag proteins as components of th Plasmodium falciparum RhopH complex

作为恶性疟原虫 RhopH 复合物成分的三种 RhopH1/Clag 蛋白的顶端表达

• 发表时间: 2005
• 期刊: Molecular and Biochemical Parasitology 143
• 作者: Kaneko 0;Yim-Lim BYS;Iriko H;Ling IT;Otsuki H;Grainger M;Tsuboi T;Adams JH;Mattei D;Holder AA;Torii M.;Kaneko O et al.
• 通讯作者: Kaneko O et al.