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Development of methods for quantitative description and perturbation of biomolecular pathways and networks

Development of methods for quantitative description and perturbation of biomolecular pathways and networks
开发生物分子途径和网络的定量描述和扰动方法
批准号:
17017004
负责人:
ITO Takashi
金额:
$66.56万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
财政年份:
2005
资助国家:
日本
项目状态:
已结题
起止时间:
2005 至 2009

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中文摘要
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英文摘要
As a basis for understanding dynamics of biomolecular pathways and networks, we developed novel methods for absolute quantification of gene expression using next generation DNA sequencing, absolute quantification of protein-protein interactions and quantitative analysis of ubiquitinated proteomes by mass spectrometry, and real-time monitoring of intracellular metabolites using FRET.
期刊论文(33)
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科研奖励(0)
会议论文
Absolute quantification of budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs
通过基因组和互补 DNA 之间的竞争性 PCR 对芽殖酵母转录组进行绝对定量
DOI: --
发表时间: 2008
期刊: BMC Genomics 9
影响因子: --
作者: [Miura, F., Kawaguchi, N., Yoshida, M., Uematsu, C., Kito, K., Sakaki, Y., Ito, T.]
通讯作者: T.
A novel, Cdc42-interacting domain of the yeast polarity establishment protein Bem1 : implications for modulation of mating pheromone signaling.
酵母极性建立蛋白 Bem1 的新型 Cdc42 相互作用结构域:对交配信息素信号传导调节的影响。
DOI: --
发表时间: 2007
期刊: J. Biol. Chem. 282
影响因子: --
作者: [Yamaguchi, Y., Ota, K., Ito, T.]
通讯作者: T.
DOI: 10.1111/j.1365-2443.2008.01228.x
发表时间: 2008-10-01
期刊: GENES TO CELLS
影响因子: 2.1
作者: [Ota, Kazuhisa, Kito, Keiji, Ito, Takashi]
通讯作者: Ito, Takashi
DOI: 10.1126/stke.4012007re6
发表时间: 2007-08
期刊: Science's STKE
影响因子: --
作者: [H. Sumimoto;S. Kamakura;Takashi Ito]
通讯作者: H. Sumimoto;S. Kamakura;Takashi Ito
22
    A novel therapeutic strategy targeting HMGB1 isoforms in sepsis
    • 批准号:
      18H02904
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.15万
    • 财政年份:
      2018
    • 负责人:
      ITO Takashi
    • 依托单位:
    A Comprehensive Analysis of Direct Democracy in the Case of Local Government and Referendums in Osaka through Repeated Cross-sectional Surveys
    Chromatin remodeling and Induction of pluripotency via Sox2/TBP complex
    • 批准号:
      17H04044
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.4万
    • 财政年份:
      2017
    • 负责人:
      ITO Takashi
    • 依托单位:
    Helicobacter pylori induces somatic mutations in TP53 via overexpression of CHAC1 in infected gastric epithelial cells
    • 批准号:
      16K19077
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.5万
    • 财政年份:
      2016
    • 负责人:
      ITO Takashi
    • 依托单位:
    海外基金