Structure and function of novel LOV proteins, LKP2 and TLP1.

新型 LOV 蛋白 LKP2 和 TLP1 的结构和功能。

• 批准号: 17084003
• 负责人: KIYOSUE Tomohiro
• 金额: $ 54.21万
• 依托单位: Gakushuin University (2008-2009)Kagawa University (2005-2007)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17084003/
• 关键词: 塊茎形成; 花芽形成; シロイヌナズナ; ジャガイモ; LKP2; LOVタンパク質; 青色光受容体; 胚軸伸長; F-boxタンパク質

We performed the characterization of Arabidopsis thaliana TLP1 as a putative blue light receptor. We showed that the involvement of auxin in the elongated hypocotyls of transgenic Arabidopsis plants that overexpressed LKP2. We also showed that both LKP2 and ZTL repressed flowering under non-inductive short-day conditions in Arabidopsis. Transgenic potato plants overexpressing Arabidopsis LKP2 formed more tubers under long-day conditions to compare with control potato plants.

我们对拟南芥TLP1作为一种假定的蓝光受体进行了表征。我们发现生长素参与过表达LKP2的转基因拟南芥植株的细长下胚轴。我们还发现LKP2和ZTL在非诱导短日照条件下抑制了拟南芥的开花。与对照马铃薯植株相比，过表达拟南芥LKP2的转基因马铃薯植株在长日照条件下形成了更多的块茎。

项目成果

Overexpression of LOV KELCH PROTEIN 2 enhances cell elongation and increases cell number and ploidy in the hypocotyl of Arabidopsis thaliana.

LOV KELCH PROTEIN 2 的过表达可增强拟南芥下胚轴中的细胞伸长并增加细胞数量和倍性。

• 发表时间: 2011
• 期刊: Plant Biotech (in press)
• 作者: Miyazaki Y.;Yoshizumi T.;Takase T.;Matsui M.;Kiyosue T.
• 通讯作者: Kiyosue T.

Promotion of tuberization by overexpression of Arabidopsis thaliana LOV KELCH REPEAT PROTEIN 2 in potato (Solanum tuberosum cv. May Queen).

通过在马铃薯（Solanum tuberosum cv. May Queen）中过度表达拟南芥 LOV KELCH REPEAT PROTEIN 2 来促进块茎形成。

• 发表时间: 2011
• 作者: Yuuki Nishiyama;Hideyuki Inui;Yasunobu Ogura,Takayoshi Shimazaki;Akira Kikuchi;Tomoyuki Takase;Tomohiro Kiyosue.
• 通讯作者: Tomohiro Kiyosue.

シロイヌナズナLOV Kelch Protein 2 (LKP2)過剰発現が及ぼす遺伝子発現変動

拟南芥 LOV Kelch 蛋白 2 (LKP2) 过度表达导致基因表达变化

• 发表时间: 2008
• 作者: 宮崎裕士;高瀬智敬;安部洋;吉積毅;松井南;小林正智;清末知宏
• 通讯作者: 清末知宏

Alternative pre-mRNA splicing events of F-box protein family in Arabidopsis

拟南芥中 F-box 蛋白家族的选择性前体 mRNA 剪接事件

• 发表时间: 2006
• 作者: Kuroda Hirofumi;Youko Horii;Tomoyuki Takase;Tomohiro Kiyosue;Minami Matsui
• 通讯作者: Minami Matsui

Anthocyanin production by over-expression of a grape transcription factor gene VlmybA2 in transgenic tobacco and Arabidopsis

通过在转基因烟草和拟南芥中过度表达葡萄转录因子基因 VlmybA2 来生产花青素

• 发表时间: 2006
• 作者: Sudarshanee Geekiyanage;Tomoyuki Takase;Tomohiro Kiyosue
• 通讯作者: Tomohiro Kiyosue