Molecular characterization of isoquinoline alkaloid biosynthesis and its reconstitution in plant and microbial cells
Molecular characterization of isoquinoline alkaloid biosynthesis and its reconstitution in plant and microbial cells
批准号:
21248013
负责人:
SATO Fumihiko
金额:
$29.7万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2012
中文摘要
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英文摘要
Isoquinoline alkaloid biosynthesis was investigated at molecular level to understand their biosynthetic pathways and its regulation. For this purpose, cDNAs of biosynthetic enzymes and transcription factors were isolated and characterized. Genes of biosynthetic enzymes isolated from C. japonicacells were successfully used to reconstitute novel alkaloid fermentation platform with some microbial enzyme genes to produce reticuline from glucose in microbial cells. In addition, bioassay system with Caenorhabditis elegans, to evaluate lipid-accumulation-reducing activity of isoquinoline alkaloids was successfully applied for the screening of new candidate among isoquinoline alkaloids.
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Caenorhabditis elegans as a screening model for biological activities of plant natural chemicals
秀丽隐杆线虫作为植物天然化学物质生物活性筛选模型
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Yit-Lai Chow, Fumihiko Sato]
通讯作者:
Fumihiko Sato
大腸菌を用いたベンジルイソキノリンアルカロイド直接発酵系の構築
苄基异喹啉生物碱大肠杆菌直接发酵体系的构建
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[中川明, 小柳喬, 南博道, 片山高嶺, 佐藤文彦, 熊谷英彦]
通讯作者:
熊谷英彦
オウレンTHBO遺伝子を導入したハナビシソウ形質転換培養細胞における代謝改変
导入 THBO 基因的奥伦西斯转基因培养细胞的代谢修饰
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[松島康高, 南 博道, 竹村知也, 佐藤文彦]
通讯作者:
佐藤文彦
Metabolic engineering and synthetic biology for the production of isoquinoline alkaloids. Chandra, S., Lata H., Varma A. (eds) Biotechnology for Medicinal Plants: Micropropagation and Improvement.
用于生产异喹啉生物碱的代谢工程和合成生物学。
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Chow, Y.L., Sato, F.]
通讯作者:
F.
イソキノリンアルカロイドの微生物発酵生産の可能性
微生物发酵生产异喹啉生物碱的可能性
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[佐藤文彦, 南博道]
通讯作者:
南博道
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A study of the historical development of the socialist children's and youth literature in the 20th century Berlin
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资助金额:$0.94万
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依托单位:
Characterizafion of gene network in isoquinoline alkaloid biosynthesis and its application
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依托单位:
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负责人:SATO Fumihiko
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依托单位:
Molecular engineering of isoquinoline alkaloid biosynthesis in higher plant using Coptis O-metheyl-transferases cDNAs
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项目类别:Grant-in-Aid for Scientific Research (B)
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依托单位:
Basic studies on the chloroplast transformation using photoautotrophic cultured cells
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负责人:SATO Fumihiko
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依托单位:
海外基金