Molecular characterization of volume-activated anion channels and elucidation of cell death-survival switching mechanisms

体积激活阴离子通道的分子表征和细胞死亡-生存转换机制的阐明

• 批准号: 21249010
• 负责人: OKADA Yasunobu
• 金额: $ 30.2万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21249010/
• 关键词: 容積感受性アニオンチャネル; マキシアニオンチャネル; 酸感受性アニオンチャネル; 細胞容積調節; アポトーシス; ネクローシス; 虚血性細胞死; ATP放出; 細胞死; TRPM7; ABCF2

In the present study, several types of anion channels, which are involved in cell death-survival switching, were investigated, and the followings were elucidated: 1. Reactive oxygen species (ROS) or dissociation of ABCF2 and protein tyrosine dephosporylation are involved in activation of VSOR and Maxi-Cl, respectively. 2. Three and two candidates were selected as the candidate molecules for VSOR and Maxi-Cl, respectively. 3. Rescue from apoptotic, necrotic and ischemic cell death is induced by regulating the activities of VSOR, ASOR and CFTR anion channels.

在本研究中，对参与细胞死亡-生存转换的几种类型的阴离子通道进行了研究，并阐明了以下内容：1.活性氧（ROS）或ABCF2的解离和蛋白质酪氨酸去磷酸化分别参与VSOR和Maxi-Cl的激活。 2.分别选择3个和2个候选分子作为VSOR和Maxi-Cl的候选分子。 3.通过调节VSOR、ASOR和CFTR阴离子通道的活性来诱导细胞凋亡、坏死和缺血性死亡的拯救。

项目成果

Protective Role of Cardiac CFTR Activation Upon Early Reperfusion Against Myocardial Infarction

• DOI: 10.1159/000341479
• 发表时间: 2012-09
• 期刊: Cellular Physiology and Biochemistry
• 作者: H. Uramoto;Toshiaki Okada;Yasunobu Okada

Volume-sensitive outwardly rectifying chloride channel in white adipocytes from normal and diabetic mice.

正常和糖尿病小鼠白色脂肪细胞中体积敏感的外向整流氯离子通道。

• 发表时间: 2010
• 期刊: Am.J.Physiol.Cell Physiol.
• 作者: H.Inoue;N.Takahashi;Y.Okada;M.Konishi
• 通讯作者: M.Konishi

Maxi-anion channel and pannexin 1 hemichannel constitute separate pathways for swelling-induced ATP release in murine L929 fibrosarcoma cells.

• DOI: 10.1152/ajpcell.00459.2011
• 发表时间: 2012-11
• 期刊: American journal of physiology. Cell physiology
• 作者: Md. Rafiqul Islam;H. Uramoto;Toshiaki Okada;R. Sabirov;Y. Okada

細胞死のチャネルメカニズムとその病態

细胞死亡通道机制及其病理

• 发表时间: 2013
• 期刊: 日本病態生理学会雑誌
• 作者: KITA;Yasuyuki;et al.;岡田泰伸
• 通讯作者: 岡田泰伸

Patch Clamp Techniques - From Beginning to Advanced Protocols

膜片钳技术 - 从初级到高级方案

• 发表时间: 2012
• 作者: 竹内久美子;設楽悦久;永松佳子;和田怜美;杉山雄一;堀江利治;岡田泰伸 (ed.)
• 通讯作者: 岡田泰伸 (ed.)