Molecular Cell Physiological Study on Paneth Cell Function

It is widely accepted that the small internal crypt is a Cl-D1-D1 secreting epithelium where CFTR CI-D1-D1 channels are thoug\to mediate cyclic AMP-dependent CI-D1 exit at the luminal？brane。实际上，CFTR mRNA在原位杂交中的一小直觉表现出了相当大的表达。Paneth细胞，在加密基础上的位置，通过保密的反微生物物质、加密蛋白和赖氨酸等方式，被隐含为多物质免疫力和宿主防御的组件。最近，渠道形成的密码被报告为提供了一种近似本地化的C1-D1行为，并为直觉CI D1-D1保密做出了贡献。However， there has been no investigation as to whether Paneth cells express CFTR C1-D1 channel。在目前的研究中，全细胞贴片钳首次被应用于几内亚猪小意图加密的Paneth细胞。Cyly D1-Cyly D1当前的活动可能只观察到与VIP或Dibutyryl循环AMP + Forskolin刺激后。The Current Characters，包括当前-电压关系、Anion选择性和DIDS不敏感性，在表观上与CFTR CI D1-D1频道的那些相似。However，两者都是单细胞RT-PCR分析和免疫化学发现失败，以提供CFTR mRNA和蛋白质分子表达的相关证据。因此，它包含了Paneth细胞快速循环AMP激活的CI'通道，但CFTR将不是几内亚猪小物体Paneth细胞中Clii D1-D1通道的分子身份。

It is widely accepted that the small internal crypt is a ClィイD1-ィエD1 secreting epithelium where CFTR CIィイD1-ィエD1 channels are thought to mediate cyclic AMP-dependent CIィイD1-ィエD1 exit at the luminal membrane. Actually, abundant expression of CFTR mRNA in the small intestine has been shown by in situ hybridization. Paneth cells, which locate at the crypt base, have been implicated as components of the mucosal immunity and host defense by secreting anti-microbial substances, such as crypidins and lysozymes. Recently, the channel-forming crypidins have been reported to serve an apically localized C1ィイD1-ィエD1 conductance, thereby contributing to the intestinal CIィイD1-ィエD1 secretion. However, there has been no investigation as to whether Paneth cells express CFTR C1ィイD1-ィエD1 channels. In the present study, whole-cell patch clamp was first applied to Paneth cells in crypts isolated from guinea pig small intestine. Prominent activation of ClィイD1-ィエD1 currents could be observed only after stimulation with VIP or dibutyryl cyclic AMP plus forskolin. The current characters, including the current-voltage relationship, anion selectivity and DIDS-insensitivity, were phenotypically similar to those of CFTR CIィイD1-ィエD1 channel. However, both single-cell RT-PCR analysis and immunocytochemical staining failed to provide relevant evidence for molecular expression of the CFTR mRNA and protein. Thus, it is concluded that Paneth cells express cyclic AMP-activated CI' channels, but CFTR would not be the molecular identity of the ClィイD1-ィエD1 channel in Paneth cells of the guinea pig small intestine.