MRM・MS and microRNA profiling analyses of blood samples and their clinical applications for development of molecular diagnostics

血液样本的 MRM・MS 和 microRNA 分析及其在分子诊断开发中的临床应用

• 批准号: 21249037
• 负责人: TAKAHASHI Takashi
• 金额: $ 27.04万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21249037/
• 关键词: 分子診断; 網羅的発現解析; プロテオミクス; マイクロRNA

MRM・MS and microRNA profiling analyses of blood samples and their clinical applications for development of molecular diagnosticsWe aimed to establish novel molecular diagnostic means for "hard-to-cure "cancers such as pancreatic cancer. We successfully identified four miRNAs present at high levels only in sera of pancreatic cancer patients but not in those of healthy individuals, which allowed us to develop diagnostic classifies for pancreatic cancers based on the expression profiles. In addition, we have successfully catalogued 40, 000 proteins from normal and pancreatic cancer tissues, which should serve as valuable basis for identification of biomarkers of pancreatic cancer. Interestingly, we identified one such protein, high expression of which appeared to confer resistance to endoplasmic reticulum stress in pancreatic cancer.

血液样本的MRM·MS和microRNA谱分析及其在分子诊断发展中的临床应用我们旨在建立胰腺癌等“难治性“癌症的新型分子诊断手段。我们成功地鉴定了仅在胰腺癌患者血清中高水平存在而在健康个体血清中不存在的四种miRNA，这使我们能够基于表达谱开发胰腺癌的诊断分类。此外，我们已经成功地从正常和胰腺癌组织中编目了40，000个蛋白质，这将为胰腺癌生物标志物的鉴定提供有价值的基础。有趣的是，我们发现了一种这样的蛋白质，其高表达似乎赋予胰腺癌对内质网应激的抵抗力。

项目成果

Roles of ASH1- regulated miRNAs and lincRNAs in lung cancer development

ASH1 调控的 miRNA 和 lincRNA 在肺癌发生中的作用

• 发表时间: 2011
• 作者: Osada H;Takahashi T
• 通讯作者: Takahashi T

Expression profiling-based approaches for a better understanding of human lung cancer biology.

基于表达谱的方法可以更好地了解人类肺癌生物学。

• 发表时间: 2009
• 作者: Takahashi;T.;Takahashi T
• 通讯作者: Takahashi T

MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis

• DOI: 10.1038/emboj.2011.416
• 发表时间: 2012-01-18
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Hosono, Yasuyuki;Yamaguchi, Tomoya;Takahashi, Takashi
• 通讯作者: Takahashi, Takashi

A Bone Metastasis Model With Osteolytic and Osteoblastic Properties of Human Lung Cancer ACC-LC-319/bone2 in Natural Killer Cell-Depleted Severe Combined Immunodeficient Mice

• DOI: 10.3727/096504009789745511
• 发表时间: 2009-01-01
• 期刊: ONCOLOGY RESEARCH
• 影响因子: 3.1
• 作者: Otsuka, Shinsaku;Hanibuchi, Masaki;Sone, Saburo
• 通讯作者: Sone, Saburo

ヒト肺癌の分子病態の発現プロファイルに基づく理解と応用

基于表达谱的人肺癌分子病理学认识与应用

• 发表时间: 2009
• 作者: Fujihara J;Yasuda T;Kawai Y;Morikawa N;Arakawa K;Koda Y;Soejima M;Kimura-Kataoka K;Takeshita H;高橋隆
• 通讯作者: 高橋隆