MRM・MS and microRNA profiling analyses of blood samples and their clinical applications for development of molecular diagnostics

MRM·MS and microRNA profiling analyses of blood samples and their clinical applications for development of molecular diagnosticsWe aimed to establish novel molecular diagnostic means for“hard-to-cure”cancers such as pancreatic cancer.We successfully identified four miRNAs present at high levels only in sera of pancreatic cancer patients but not in those of healthy individuals，which allowed us to develop diagnostic classifies for pancreatic cancers based on the expression profiles。In addition，we have successfully catalogued 40，000 proteins from normal and pancreatic cancer tissues，which should serve as valuable basis for identification of biomarkers of pancreatic cancer.Interestingly，we identified one such protein，high expression of which appeared to confer resistance to endoplasmic reticulum stress in pancreatic cancer。

MRM・MS and microRNA profiling analyses of blood samples and their clinical applications for development of molecular diagnosticsWe aimed to establish novel molecular diagnostic means for "hard-to-cure "cancers such as pancreatic cancer. We successfully identified four miRNAs present at high levels only in sera of pancreatic cancer patients but not in those of healthy individuals, which allowed us to develop diagnostic classifies for pancreatic cancers based on the expression profiles. In addition, we have successfully catalogued 40, 000 proteins from normal and pancreatic cancer tissues, which should serve as valuable basis for identification of biomarkers of pancreatic cancer. Interestingly, we identified one such protein, high expression of which appeared to confer resistance to endoplasmic reticulum stress in pancreatic cancer.