Development of Compact Diffracted X-ray Tracking Instrumentation

在2000年，我们成功地进行了体外时间分辨X射线观察的皮米级缓慢布朗运动的单个蛋白质分子在水溶液中。在这个单分子检测系统中，我们称之为衍射X射线跟踪（DXT），我们通过使用时间分辨劳厄衍射技术观察到单个蛋白质分子中标记到特定位点的单个双链体的旋转运动。这是本研究的目的，我们进行紧凑的DXT仪器，使测量的单分子的这种高精度的追求方法在实验室水平。有三个技术要点需要实现。我们成功地制作了新的X射线管，X射线浓缩和背景还原。

In 2000, we successfully conducted in vitro time-resolved X-ray observations of picometer-scale slow Brownian motions of individual protein molecules in aqueous solutions. In this single-molecule detection system, which we call diffracted X-ray tracking (DXT), we observed the rotating motions of an individual nanocrystal labeled to a specific site in individual protein molecules by using the time-resolved Laue diffraction technique. It is the purpose of this research that we perform the compact DXT instrumentation which enables measurement of the single molecule of this high precision pursuing method on a laboratory level. There were three technical points for realization. We succeeded in the new nanocrystal’s production, x-ray condensing, and a background’s reduction.