Development of Compact Diffracted X-ray Tracking Instrumentation

2000年，我们成功地对单个蛋白质分子在水溶液中的皮米尺度慢布朗运动进行了体外时间分辨X射线观测。在这个我们称为衍射X射线跟踪(DXT)的单分子检测系统中，我们利用时间分辨的劳厄衍射技术观察到了单个纳米晶体标记到单个蛋白质分子中特定位置的旋转运动。这项研究的目的是我们进行紧凑的DXT仪器，使这种高精度追踪法的单分子测量能够在实验室水平上进行。要实现这一点有三个技术要点。我们成功地完成了新纳米晶体的生产、X射线凝聚和背景还原。

In 2000, we successfully conducted in vitro time-resolved X-ray observations of picometer-scale slow Brownian motions of individual protein molecules in aqueous solutions. In this single-molecule detection system, which we call diffracted X-ray tracking (DXT), we observed the rotating motions of an individual nanocrystal labeled to a specific site in individual protein molecules by using the time-resolved Laue diffraction technique. It is the purpose of this research that we perform the compact DXT instrumentation which enables measurement of the single molecule of this high precision pursuing method on a laboratory level. There were three technical points for realization. We succeeded in the new nanocrystal’s production, x-ray condensing, and a background’s reduction.