Integrated molecular analysis of taste signal generation, transmission and cognition

研究概要：在味觉系统的综合分析中，(1)我们找到了参与咸味感受(I型)细胞膜电位构建的靶基因Ano1、Kene3、Sec61a1：(2)PLC-Beta2-WGA Medaka FISH分析全面阐明了苦味/氨基酸感受细胞到终脑的味觉传递途径，并将感受咸味的细胞连续可视化到PKD1L3-WGA小鼠的NST味觉区域；3SNAP25、EGR-2和KROX-20等断奶小鼠在断奶期间给予固体食物后，其味觉区域的信号明显被激活，而在继续给予液体食物的情况下，SNAP25、EGR-2和KROX-20在味觉区域的信号被激活。(4)食物的口感质量与食物摄入量之间存在一定的关系。

Outline of the research: For the integrated analysis of taste systems, (1) we found the target genes AnoI, Kene3, Sec61a1 that are involved in construction of electric potential of saltiness reception (type I) cell membrane; (2) PLC-beta2-WGA medaka fish analysis led to comprehensive elucidation of the taste transmission pathway from bitterness/amino acid receiving cells to the endbrain and the visualization of saltiness-receiving cell to the NST taste area in PKD1L3-WGA mice was successive; and (3) integrated expression markers in weaning mice, SNAP25, EGr-2 and Krox-20, were distinctly activated its signal in the taste area when the mice were given a solid food at the stage of weaning in comparison with the case that they were still given a liquid food. (4) we also showed that there is a relationship between the tastant quality in diet and the food intake.