Integrated molecular analysis of taste signal generation, transmission and cognition

研究概要：在味觉系统的综合分析中，（1）发现了与盐感受（I型）细胞膜电位构建相关的目的基因AnoI、Kene 3、Sec 61 a1;（2）PLC-beta2-WGA青鳉鱼分析导致全面阐明了从苦味/氨基酸接收细胞到端脑的味觉传递途径和咸味的可视化。（3）断奶期给予固体食物的小鼠与仍给予液体食物的小鼠相比，其味觉区整合表达标志物SNAP 25、EGr-2和Krox-20的信号明显激活。(4)我们还表明，在饮食中的促味剂质量和食物摄入量之间存在关系。

Outline of the research: For the integrated analysis of taste systems, (1) we found the target genes AnoI, Kene3, Sec61a1 that are involved in construction of electric potential of saltiness reception (type I) cell membrane; (2) PLC-beta2-WGA medaka fish analysis led to comprehensive elucidation of the taste transmission pathway from bitterness/amino acid receiving cells to the endbrain and the visualization of saltiness-receiving cell to the NST taste area in PKD1L3-WGA mice was successive; and (3) integrated expression markers in weaning mice, SNAP25, EGr-2 and Krox-20, were distinctly activated its signal in the taste area when the mice were given a solid food at the stage of weaning in comparison with the case that they were still given a liquid food. (4) we also showed that there is a relationship between the tastant quality in diet and the food intake.