The effect of sex hormone on canilaae metabolism

性激素对软骨代谢的影响尚不清楚，雌激素对软骨分化的影响也鲜有报道。在成人的骨重建过程中，雌激素对于维持成骨细胞和破骨细胞活动之间的平衡是必不可少的。为探讨雌激素促进成软骨细胞分化的机制，采用ATDC5细胞和原代培养的小鼠肋骨成软骨细胞进行体外实验。Alcian蓝染色检测软骨结节，实时逆转录聚合酶链式反应检测II型胶原基因表达。在雌激素处理的细胞中进行X型胶原和琼脂糖胶原染色。作为软骨分化标志的聚集素和II型胶原在细胞中的表达水平在低剂量雌激素(10^~(-12)-12~gt；M)作用下持续升高，而在高剂量(10^~(-6)&gt；M)作用下被抑制。ATDC5和经一定浓度雌激素处理的原代培养的软骨细胞具有较高的Col II和aggrecan表达。

The effect of sex hormone on cartilage metabolism is unclear, and little has been reported about the effects of estrogen on the cartilage differentiation. Estrogen is essential for maintaining the balance between osteoblastic and osteoclastic activity during bone remodeling in adults. To investigate the mechanism by which estrogen enhances chondrogenic differentiation, in vitro experiments were carried out using ATDC5 cells(a mouse pre-chondrogenic cell line) and primary cultured chondrogenic cells of mouse rib. Alcian blue staining for cartilage nodules and real-time reverse-transcription polymerase chain reaction for the expression of genes encoding type II collagen. type X collagen and augrecan, were performed in cells treated with estrogen. The expression level of aggrecan and type II collagen in cells, a marker of chondrogenic differentiation, was consistently elevated by low dose of estrogen(10^<-12> M) and was suppressed by high dose(10^<-6> M). ATDC5 and primary cultured chondrogenic cells treated with certain concentration of estrogen have higher expression of Col II and aggrecan.