Analyse von Proteinen, die mit der nukleären, Ras-ähnlichen GTPase Ran in Saccharomyces cerevisiae interagieren

酿酒酵母中与核 Ras 样 GTPase Ran 相互作用的蛋白质分析

• 批准号: 5107048
• 负责人: Dr. Markus Künzler
• 金额: --
• 依托单位: Institut für Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1998
• 资助国家: 德国
• 起止时间: 1997-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5107048?language=en
• 关键词: Analyse; von; Proteinen; die; mit

The nuclear Ras-like GTPase Ran has been implicated in a wide variety of cellular processes, including nucleocytoplasmic transport, cell cycle progression, maintenance of chromatin and nuclear organization, nucleolar structure, DNA replication, chromosome segregation, RNA metabolism, microtubule organization, and yeast mating. A direct function for Ran and some of its interacting factors, however, has so far only been established for nucleocytoplasmic transport. It is therefore unclear whether the diversity of processes affected by Ran and its associated factors is a indirect consequence of their primary role in nucleocytoplasmic transport, or is due to multiple functions of Ran in the cell. In order to reveal the molecular basis of Ran's functional diversity we are analyzing the cellular function of Ran-interacting proteins in the yeast Saccharomyces cerevisiae. Besides a functional analysis of established or suggested Ran-interacting proteins, we aim at the identification and characterization of novel representatives of such proteins in this organism.

核Ras样GTP酶RAN参与了多种细胞过程，包括核质转运、细胞周期进程、染色质和核组织的维持、核仁结构、DNA复制、染色体分离、RNA代谢、微管组织和酵母交配。然而，到目前为止，RAN及其一些相互作用因子的直接作用仅被建立在核质运输上。因此，目前尚不清楚RAN及其相关因素影响的过程的多样性是它们在核质运输中的主要作用的间接结果，还是由于RAN在细胞中的多种功能所致。为了揭示Ran功能多样性的分子基础，我们分析了酿酒酵母中Ran相互作用蛋白的细胞功能。除了对已建立的或建议的Ran相互作用蛋白进行功能分析外，我们的目标是鉴定和表征这类蛋白在该生物体中的新代表。