Regulation of heat shock response, maltose transport, pyrimidine synthesis and ribosomal RNA synthesis in Pyrococcus furiosus

激烈火球菌热休克反应、麦芽糖转运、嘧啶合成和核糖体 RNA 合成的调节

• 批准号: 5132026
• 负责人: Professor Dr. Michael Thomm
• 金额: --
• 依托单位: Lehrstuhl für Mikrobiologie und Archaeenzentrum
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5132026?language=en
• 关键词: Regulation; heat; shock; response; maltose

Two transcriptional regulators of Pyrococcus have been characterized preliminarily in this laboratory. The Leucine responsive regulatory protein (LRP) and a 24 kDa regulator of a heat shock regulon. The interaction of these components with the basal transcriptional machinery and their mechanism of action will be studied in molecular detail. DNA-protein interactions will be investigated by various footprinting techniques, protein-protein interactions will be studied in vitro and in vivo. The crystal structure of the heat shock regulator both alone and in complex with its target DNA will be determined. The effect of NTP concentration on transcription of various promoters directing expression of proteins involved in pyrimidine synthesis will be analysed. *

本实验室对火球菌属的两个转录调节子进行了初步的鉴定。亮氨酸应答调节蛋白（LRP）和热休克调节子的24 kDa调节剂。这些组件的相互作用与基本的转录机制和它们的作用机制将在分子详细研究。将通过各种足迹技术研究DNA-蛋白质相互作用，将在体外和体内研究蛋白质-蛋白质相互作用。将确定单独的和与其靶DNA复合的热休克调节剂的晶体结构。将分析NTP浓度对指导参与嘧啶合成的蛋白质的表达的各种启动子的转录的影响。 *