Cell Sorter

我们特此申请采购一种易于使用的电池分选机，作为一种台式设备，它可以方便地为不同的应用程序分拣各种尺寸的电池。我们是一群年轻的创新研究小组，拥有图宾根大学的基本资金，也成功地获得了欧洲研究理事会或德国研究基金会的资金。事实上，我们所有人都在国外待了很长一段时间(纪念斯隆·凯特琳癌症中心的Feucht&Leibold，斯坦福大学的Schürch，以及国家卫生研究院的Flatz)。除了免疫学，我们还因为接受了图宾根的一个职位而团结在一起，我们的愿景是通过基础和翻译研究决定性地推进我们的领域。在这样做的过程中，作为临床科学家，我们一再成功地承担起在人体样本基础研究中验证我们的发现的任务，从而使研究更接近临床或患者的需求。从我们的日常临床工作中，我们了解到目前诊断和治疗的局限性。由于我们在很大程度上都在处理难以获得的人类样本，因此我们必须日夜直接接触细胞分选设备，并在周末直接接触到可以由我们的实验室成员独立操作的细胞分选设备。较短的距离还可以确保样品保持新鲜，这样它们就可以在进行过程中进行扩展，并可用于进一步的实验。因此，仪器的一个重要标准是尽可能地完全自动化设置、采集、分类和分析，以便分类易于执行，从而独立于操作员。系统软件应该是直观的，并支持对管子和96孔板和384孔板进行分类。原始分拣数据应在FCS 3.0和FCS 3.1文件中可用，以便可以将其导出到第三方分析工具。所描述的两个设备符合这些规范。获得这样的设备将大大加快收集新数据的速度，并改善团队之间的合作。这样的设备将帮助我们进行与世界领先实验室不相上下的实验，并巩固在图宾根的研究地点。这对于未来招募更多有才华的年轻研究人员也是至关重要的。

We hereby apply for the procurement of a user-friendly cell sorter which, as a benchtop device, enables the easy sorting of a wide range of cell sizes for diverse applications. We are a cluster of young and innovative research groups that have basic funding from the University of Tübingen and have also been successful in obtaining funding from the European Research Council or the German Research Foundation. Virtually all of us have been abroad for extended periods of time (Feucht & Leibold at Memorial Sloan Kettering Cancer Center, Schürch at Stanford University, and Flatz at the National Institutes of Health). In addition to immunology, we are united by the fact that we accepted a position in Tübingen with the vision of decisively advancing our fields through basic and translational research. In doing so, as clinician-scientists, we repeatedly successfully take on the task of verifying our discoveries in basic research on human samples, thus bringing research closer to the needs of the clinic or the patient. From our daily clinical work, we know the current limitations of diagnostics and therapeutics. Since we all work to a large extent with human samples that are difficult to obtain, it is crucial that we have direct access day and night and on weekends to a cell sorting device that can be operated independently by our laboratory members. A short distance also ensures that the samples remain fresh so that they can be expanded as they progress and are available for further experiments. Therefore, an important criterion for the instrument is to automate setup, acquisition, sorting, and analysis as completely as possible so that sorting is easy to perform and thus independent of an operator. The system software should be intuitive and support sorting into tubes and 96- and 384-well plates. Raw sorting data should be available in FCS 3.0 and FCS 3.1 files so that they can be exported to third-party analysis tools. These specifications are met by the two devices described. Obtaining such a device will greatly speed up the collection of new data and improve collaboration among teams. Such equipment will help us to perform experiments on par with world-leading laboratories and consolidate the research location in Tübingen. This will also be crucial for recruiting more talented young researchers in the future.