Development of mechanistic probes for the detection of radical and radical ionic intermediates in enzymatic reactions

开发用于检测酶反应中自由基和自由基离子中间体的机械探针

• 批准号: 5177286
• 负责人: Professor Dr. Michael Schmittel
• 金额: --
• 依托单位: Arbeitsgruppe Organische Chemie I
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5177286?language=en
• 关键词: Development; mechanistic; probes; detection; radical

In several coenzyme B12 catalyzed eliminase reactions, such as the Ribonucleotide Reductase, the Propanediol and Glycerol dehydratase and the Ethanolamine Ammonia Lyase, neutral and ionic radicals have been invoked as mechanistically important intermediates. While neutral radicals can in principle be tested using radical clock reactions, there is no reliable tool yet to probe for the intermediacy of short-lived ion radicals. Hence, it is proposed to develop a new class of electron transfer probes that allow to recognize radical ionic intermediates directly in enzymatic processes by using product analysis studies and EPR detection.

在几种辅酶B12催化的消除酶反应中，如核糖核苷酸还原酶、丙醇和甘油脱氢酶和乙醇胺氨裂解酶，中性和离子自由基被用作重要的机械中间体。虽然中性自由基原则上可以使用自由基时钟反应进行测试，但目前还没有可靠的工具来探测短寿命离子自由基的中介作用。因此，它建议开发一类新的电子转移探针，允许识别自由基离子中间体直接在酶促过程中，通过使用产品分析研究和EPR检测。