NMR STUDY OF SMALL HEAT SHOCK PROTEIN (HSP12) FROM CESG

来自 CESG 的小热休克蛋白 (HSP12) 的 NMR 研究

• 批准号: 8361187
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 0.02万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361187
• 关键词: Buffers; Data; Dehydration; Detergents; Funding; Grant; Heat shock proteins; Heat-Shock Response; Membrane; Methods; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; Structure; United States National Institutes of Health; cost; protein folding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NMR study of small heat shock unfolded protein (HSP12) from CESGHSP12 is a small heat shock protein, which protects the membrane from dehydration and is unfolded in regular NMR buffer. Priliminary studies indicate this protein folds in presence of detergents (SDS). we are planning to determine the structure in presence of SDS. In addition we are using HIFI fast data method to assign the protein.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 小热休克未折叠蛋白 (HSP12) 的 NMR 研究 来自 CESGHSP12 是一种小热休克蛋白，可保护膜免于脱水，并在常规 NMR 缓冲液中展开。初步研究表明该蛋白质在去垢剂 (SDS) 存在下会折叠。我们计划在 SDS 存在的情况下确定结构。此外，我们使用 HIFI 快速数据方法来分配蛋白质。