NMR STUDY OF SMALL HEAT SHOCK PROTEIN (HSP12) FROM CESG

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 CESGHSP 12中小分子热休克未折叠蛋白（HSP 12）的NMR研究CESGHSP 12是一种小分子热休克蛋白，保护膜不受脱水，在常规NMR缓冲液中未折叠。初步研究表明，这种蛋白质在洗涤剂（SDS）的存在下折叠。我们计划在SDS存在的情况下确定结构。此外，我们使用HIFI快速数据方法来分配蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. NMR study of small heat shock unfolded protein (HSP12) from CESGHSP12 is a small heat shock protein, which protects the membrane from dehydration and is unfolded in regular NMR buffer. Priliminary studies indicate this protein folds in presence of detergents (SDS). we are planning to determine the structure in presence of SDS. In addition we are using HIFI fast data method to assign the protein.