NMR STUDY OF SMALL HEAT SHOCK PROTEIN (HSP12) FROM CESG

来自 CESG 的小热休克蛋白 (HSP12) 的 NMR 研究

• 批准号: 8169009
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 0.45万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169009
• 关键词: Buffers; Computer Retrieval of Information on Scientific Projects Database; Data; Dehydration; Detergents; Funding; Grant; Heat shock proteins; Heat-Shock Response; Institution; Membrane; Methods; Proteins; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; protein folding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. NMR study of small heat shock unfolded protein (HSP12) from CESGHSP12 is a small heat shock protein, which protects the membrane from dehydration and is unfolded in regular NMR buffer. Priliminary studies indicate this protein folds in presence of detergents (SDS). we are planning to determine the structure in presence of SDS. In addition we are using HIFI fast data method to assign the protein.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 小热休克未折叠蛋白 (HSP12) 的 NMR 研究 来自 CESGHSP12 是一种小热休克蛋白，可保护膜免于脱水，并在常规 NMR 缓冲液中展开。初步研究表明该蛋白质在去垢剂 (SDS) 存在下会折叠。我们计划在 SDS 存在的情况下确定结构。此外，我们使用 HIFI 快速数据方法来分配蛋白质。