Genotypic classification of GB virus C/Hepatitis G virus by molecular evolutionary analysis and its clinical application

1996年在西非和美国分别从人血清中分离到新的非A-E型肝炎候选病毒GB病毒C（GBV-C）和G型肝炎病毒（HGV）。由于发现它们之间的氨基酸序列约90%同源，因此它们被认为是同一种病毒，但基因型不同。GBV-C/HGV被认为是引起急性和慢性肝炎，最终通过输血发展为肝细胞癌（HCC）和荧光性肝炎。采用聚合酶链反应法对全球2000例不同肝病患者的GBV-C/HGV RNA进行检测。GBV-C/HGV RNA阳性与特定肝脏疾病之间没有关联。采用限制性片段长度多态性（RFLP）方法建立了GBV-C/HGV基因分型方法，并调查了基因型在世界范围内的流行情况。尽管基因型与特定肝脏疾病之间没有关联，但亚洲型和肝细胞癌伴HBV阳性之间存在显著关联。由于已有报道称，在亚洲，p53点突变与HCC与HBV有很强的相关性，我们现在正在检查HBV阳性或亚洲型GBV-C/HGV HCC患者中p53的核苷酸序列。

New candidate viruses for non A-E hepatitis virus were isolated from human sera in west Africa, designated GB virus C(GBV-C) and in USA, designated hepatitis G virus (HGV) in 1996. As they were found about 90% homology in amino acid sequences between them, they were thought to be a same virus and different genotypes. GBV-C/HGV has been supposed to cause acute and chronic hepatitis, and finally develop to hepatocellular carcinoma (HCC) and also fluminant hepatitis through blood transfusion.We investigate GBV-C/HGV RNA by polymerase chain reaction method among 2,000 samples with various liver diseases in the world. There were no association between the positivity of GBV-C/HGV RNA and a specific liver disease. We developed the GBV-C/HGV genotyping method by restriction fragment length polymorphism(RFLP) method and investigated the prevalence of genotypes in the world. There was a significant association between the positivity of Asian type and hepatocellular carcinoma with HBV, although there was no association between the genotype and a specific liver disease. As it has been already reported the strong association between the point mutation in p53 and HCC with HBV in Asia, we are now checking the nucleotide sequences in p53 among the HCC patients both positive or HBV and Asian type of GBV-C/HGV.