Genotypic classification of GB virus C/Hepatitis G virus by molecular evolutionary analysis and its clinical application

丙型肝炎病毒/庚型肝炎病毒的分子进化分析基因型分类及其临床应用

• 批准号: 09670563
• 负责人: MIZOKAMI Masashi
• 金额: $ 1.98万
• 依托单位: NAGOYA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670563/
• 关键词: GBV-C; HGV; molecular evolutionary; genotype; RFLP; hepatocellular carcinoma; RELP; 癌抑制遺伝子; 肝炎ウイルス; G型肝炎ウイルス; Genotype; polymerase chair reaction

New candidate viruses for non A-E hepatitis virus were isolated from human sera in west Africa, designated GB virus C(GBV-C) and in USA, designated hepatitis G virus (HGV) in 1996. As they were found about 90% homology in amino acid sequences between them, they were thought to be a same virus and different genotypes. GBV-C/HGV has been supposed to cause acute and chronic hepatitis, and finally develop to hepatocellular carcinoma (HCC) and also fluminant hepatitis through blood transfusion.We investigate GBV-C/HGV RNA by polymerase chain reaction method among 2,000 samples with various liver diseases in the world. There were no association between the positivity of GBV-C/HGV RNA and a specific liver disease. We developed the GBV-C/HGV genotyping method by restriction fragment length polymorphism(RFLP) method and investigated the prevalence of genotypes in the world. There was a significant association between the positivity of Asian type and hepatocellular carcinoma with HBV, although there was no association between the genotype and a specific liver disease. As it has been already reported the strong association between the point mutation in p53 and HCC with HBV in Asia, we are now checking the nucleotide sequences in p53 among the HCC patients both positive or HBV and Asian type of GBV-C/HGV.

1996年在西非和美国分别从人血清中分离出了新的非甲-戊型肝炎候选病毒，命名为GB病毒C（GBV-C）和庚型肝炎病毒（HGV）。由于氨基酸序列同源性达90%左右，推测它们是同一种病毒，但基因型不同。GBV-C/HGV可引起急、慢性肝炎，通过输血可发展为肝细胞癌（HCC）和流行性肝炎，本文采用聚合酶链反应（PCR）方法对世界各地2,000例不同肝病患者的血清中GBV-C/HGV RNA进行了检测。GBV-C/HGV RNA阳性与特定肝病之间无相关性。本研究采用限制性片段长度多态性（RFLP）技术建立了GBV-C/HGV基因分型方法，并调查了世界范围内GBV-C/HGV基因型的流行情况。虽然基因型与特定的肝脏疾病之间没有关联，但亚洲型阳性与HBV感染的肝细胞癌之间存在显著关联。由于已经报道了p53点突变与亚洲HCC和HBV之间的强相关性，我们现在正在检查HCC患者中p53的核苷酸序列，无论是HBV阳性还是亚洲型GBV-C/HGV。

项目成果

Etsuro Orito: "Interferon-α therapy in patients dually infected with hepatitis C virus and GB virus C/hepatitis G virus-virological response of HGV and pretreatment HGV viremia level" Journal of Hepatology. 27. 603-612 (1997)

Etsuro Orito：“丙型肝炎病毒和 GB 病毒 C/G 型肝炎病毒双重感染患者的干扰素-α 治疗 - HGV 病毒学反应和治疗前 HGV 病毒血症水平”《肝脏病学杂志》27. 603-612 (1997)。

Yasuhito Tanaka: "GB virus C/hepatitis G virus infection among patients with hepatocellular carcinoma" -8. 44-51 (1997)

Yasuhito Tanaka：“肝细胞癌患者中的GB病毒C/G型肝炎病毒感染”-8。

Nakano T: "TT virus infection among blood donors and patients with non-B non-C liver diseases in Korea" Journal of Hepatology. 30(3). 389-393 (1999)

Nakano T：“韩国献血者和非 B 非 C 肝病患者中的 TT 病毒感染”《肝脏病学杂志》。

Masashi Mizokami: "Constrained Evolution with Respect to Geno Overlap Hepatitis B Virus" Journal of Molecular Evolution. 44. S83-S90 (1997)

Masashi Mizokami：“关于基因组重叠乙型肝炎病毒的受限进化”分子进化杂志。

Takanobu kato: "High prevalence of GB virus C/hepatitis G virus infection among the Jewish population in Uzbekistan" Virus Research. 48. 81-87 (1997)

Takanobu Kato：“乌兹别克斯坦犹太人口中 GB 病毒 C/G 型肝炎病毒感染的高流行”病毒研究。