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HBV genotype distribution in Japan and it's clinical significance

日本HBV基因型分布及其临床意义

基本信息

批准号：
12670506
负责人：
MIZOKAMI Masashi
金额：
$ 2.43万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670506/
关键词：
HBV genotype distribution diagnosis prognosis Genotype A Genotype B Genotype C HBe抗原分布状況

项目摘要

The geographic distribution of HBV genotypes in Japan and its clinical relevance are poorly understood. 720 Japanese patients with chronic HBV infection was determined HBV genotype by PCR-RFLP. 1.7 %, 12.2 %, 84.7 %, 0.4 %, 1.0 % were classified into HBV genotype A, B, C, D and mixed. 94.2 % of patients in the Japan mainland had genotype C, while 60.0 % of the patients in Okinawa and 22.9 % in the Tohoku, the northern part of mainland, harbored genotype b. Compared with genotype C patients, genotype B patients showed elder age, lower rate of positive HBeAg, lower level of serum HBV DNA. These results indicate tat genotypes C and B are predominant in Japan, and there are significant differences in geographic distribution and clinical characteristics among the patients with the different genotypes. The clinical and virological differences of HBV genotypes B and C are unclear. To evaluate the differences of HBV genotype B and C, we carried out a case-control study in 50 each of Japanese patients chronically infected with HBV genotype B and C, who were matched for age and sex. HBeAg was more frequently detected, while anti-HBe was less common in genotype B than C patients. The occurrence of A-to-G mutation at nt 1896 in the precore region was comparable between genotype B and C patients, and it correlated with anti-HBe. The double mutation in core promoter was more frequent in genotype C than B patients, and did not correlate with HBeAg. By multiple logistic regression analysis, double mutations in core promoter was associated significantly with genotype C, age 35 years and more advanced liver disease, but it was not associated with sex, HBeAg, HBV DNA or the precore mutation. These results highlights differences in clinical and virological characteristics between patients infected with HBV of genotypes B and C.

日本 HBV 基因型的地理分布及其临床相关性尚不清楚。采用PCR-RFLP方法对720例日本慢性HBV感染患者进行了HBV基因型测定。 1.7%、12.2%、84.7%、0.4%、1.0%分为HBV基因型A、B、C、D和混合型。日本本土94.2%的患者携带C基因型，而冲绳60.0%的患者和东北地区22.9%的患者携带b基因型。与C基因型患者相比，B基因型患者年龄较大，HBeAg阳性率较低，血清HBV DNA水平较低。这些结果表明tat基因型C和B在日本占主导地位，不同基因型患者的地理分布和临床特征存在显着差异。 B 型和 C 型 HBV 的临床和病毒学差异尚不清楚。为了评估 B 型和 C 型 HBV 的差异，我们对 50 名慢性感染 B 型和 C 型 HBV 的日本患者进行了病例对照研究，这些患者的年龄和性别相匹配。与 C 基因型患者相比，B 基因型患者检测到 HBeAg 的频率更高，而抗 HBe 检测的频率较低。 B 型和 C 型患者的前核心区 nt 1896 处 A 至 G 突变的发生率相当，并且与抗 HBe 相关。核心启动子双突变在C基因型患者中比B基因型患者更常见，并且与HBeAg无关。通过多重logistic回归分析，核心启动子的双突变与C基因型、年龄35岁和更严重的肝病显着相关，但与性别、HBeAg、HBV DNA或前核心突变无关。这些结果凸显了感染 B 型和 C 型 HBV 的患者之间临床和病毒学特征的差异。