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HBV genotype distribution in Japan and it's clinical significance

日本HBV基因型分布及其临床意义

基本信息

批准号：
12670506
负责人：
MIZOKAMI Masashi
金额：
$ 2.43万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670506/
关键词：
HBV genotype distribution diagnosis prognosis Genotype A Genotype B Genotype C HBe抗原分布状況

项目摘要

The geographic distribution of HBV genotypes in Japan and its clinical relevance are poorly understood. 720 Japanese patients with chronic HBV infection was determined HBV genotype by PCR-RFLP. 1.7 %, 12.2 %, 84.7 %, 0.4 %, 1.0 % were classified into HBV genotype A, B, C, D and mixed. 94.2 % of patients in the Japan mainland had genotype C, while 60.0 % of the patients in Okinawa and 22.9 % in the Tohoku, the northern part of mainland, harbored genotype b. Compared with genotype C patients, genotype B patients showed elder age, lower rate of positive HBeAg, lower level of serum HBV DNA. These results indicate tat genotypes C and B are predominant in Japan, and there are significant differences in geographic distribution and clinical characteristics among the patients with the different genotypes. The clinical and virological differences of HBV genotypes B and C are unclear. To evaluate the differences of HBV genotype B and C, we carried out a case-control study in 50 each of Japanese patients chronically infected with HBV genotype B and C, who were matched for age and sex. HBeAg was more frequently detected, while anti-HBe was less common in genotype B than C patients. The occurrence of A-to-G mutation at nt 1896 in the precore region was comparable between genotype B and C patients, and it correlated with anti-HBe. The double mutation in core promoter was more frequent in genotype C than B patients, and did not correlate with HBeAg. By multiple logistic regression analysis, double mutations in core promoter was associated significantly with genotype C, age 35 years and more advanced liver disease, but it was not associated with sex, HBeAg, HBV DNA or the precore mutation. These results highlights differences in clinical and virological characteristics between patients infected with HBV of genotypes B and C.

日本HBV基因型的地理分布及其临床相关性知之甚少。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对720例日本慢性HBV感染患者进行HBV基因型检测。1.7 HBV基因型分为A、B、C、D型和混合型分别占12.2%、84.7%、0.4%、1.0%。94.2在日本大陆，%的患者具有C基因型，而在冲绳和东北（大陆的北方部分），60.0%的患者具有B基因型。与C基因型患者相比，B基因型患者年龄大，HBeAg阳性率低，血清HBV DNA水平低。这些结果表明达特基因型C和B在日本占优势，不同基因型患者的地理分布和临床特征存在显著差异。HBV基因型B和C的临床和病毒学差异尚不清楚。为了评估HBV基因型B和C的差异，我们对年龄和性别匹配的50例日本HBV基因型B和C慢性感染患者进行了病例对照研究。基因型B患者中HBeAg检出率高于C型患者，而抗-HBe检出率低于C型患者。基因型B和C患者前C区1896位碱基A → G突变的发生率相似，且与抗-HBe相关。C基因型患者核心启动子区双突变发生率高于B基因型患者，且与HBeAg无关。多因素Logistic回归分析显示，C基因型、年龄35岁、晚期肝病与核心启动子双突变显著相关，而与性别、HBeAg、HBV DNA及前C区突变无关。这些结果突出了感染基因型B和C的HBV患者在临床和病毒学特征上的差异。