Molecular Biological Study of Initial Acquisition of Mutans Streptococci in Children's Mouths

儿童口腔中变形链球菌初次获得的分子生物学研究

• 批准号: 09672108
• 负责人: KOZAI Katsuyuki
• 金额: $ 1.98万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672108/
• 关键词: MUTANS STREPTOCOCCI; TRANSMISSION; DNA FINGERPRINTING; INITIAL ACOUISITION; DENTAL CARIES; 家族内伝播; 乳幼児; 歯牙萌出; 菌定着

The aims of this study are to find out the initial acquisition of mutans streptococci (MS) in chilren's oral mouths and the source of infection and to observe intrafamilial distribution longitudinaly. Twenty of the 35 children initially acquired MS at a median age of 24.5 months. The rest of the samples remained free from MS mainly the children with primary teeth have not erupted yet. The colonies count showed numbers of colonies in each child increase from time to time. MS acquision is sooner in child has not spaced dentitional arch (ave. 1y7m) than in child has not spaced dentitional arch (2y3m). DNA fingerprinting result showed that mother is not considered to be the main source of infection, some children appeared to acquire mutans from someone else. In longitudinal study of intrafamilial MS distribution for three families, MS strains were variable among 4 years and new strains were derived chiefly from familiy members.

本研究旨在了解儿童口腔中变形链球菌（MS）的初始感染情况及感染源，并纵向观察其家族内分布。35名儿童中有20名最初在24.5个月的中位年龄获得多发性硬化症。其余样本均未发现MS，主要为乳牙未长出的儿童。菌落计数显示每个子代的菌落数量不断增加。没有牙弓间隔的儿童（年龄为1y7米）比没有牙弓间隔的儿童（年龄为2y3米）更快获得MS。DNA指纹分析结果表明，母亲不被认为是主要的感染源，一些儿童似乎从其他人那里获得了突变体。在对3个家族的家族内MS分布的纵向研究中发现，MS菌株在4年内是可变的，新菌株主要来源于家族成员。

项目成果

桑原さつき: "ミュータンスレンサ球菌の家族内分布に関する経年的研究" 小児歯科学雑誌. 35・2. 340 (1997)

Satsuki Kuwabara：“变形链球菌家族分布的纵向研究”《儿童牙科杂志》35・2（1997）。

Katsuyuki Kozai: "Intratamilial Distribution of Mutans Streptococci in Japanese Families and Possibility of Father-to-Child Transmission" Microbiology and Immunology. 43・2. (1999)

Katsuyuki Kozai：“日本家庭中变异链球菌的家庭内分布和父子传播的可能性”微生物学和免疫学（1999）。

SATSUKI KUWAHARA: "A LONGITUDINAL STUDY OF INTRAFAMILIAL DISTRIBUTION OF MUTANS STREPTOCOCCI" JAP J PED DENT. 35. 340

Satsuki kuwahara：“变形链球菌家族内分布的纵向研究”JAP J PED DENT。

KATSUYUKI KOZAI: "INTRAFAMILIAL DISTRIBUTION OF MUTANS STREPTOCOCCI IN JAPANESE FAMILIES AND POSSIBILITY OF FATHER-TO CHILD TRANSMISSION" MICROBIO IMMUNOL. 43. (1999)

KATSUYUKI KOZAI：“日本家庭中变形链球菌的家庭内分布和父子传播的可能性”微生物免疫。

Katsuyuki Kozai: "Intrafamilial Distribution of Mutans Streptococci in Japanese Families and Possibility of Father-to-Child Transmission" Microbiology and Immunology. 43・2. (1999)

Katsuyuki Kozai：“日本家庭中变形链球菌的家庭分布和父子传播的可能性”微生物学和免疫学（1999）。