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ANALYSIS OF INTRAFAMILIAL TRANSMISSION OF MUTANS STREPTOCOCCI USING DNA FINGERPRINTING

使用 DNA 指纹分析变形链球菌的家庭内传播

基本信息

批准号：
05671711
负责人：
KOZAI Katsuyuki
金额：
$ 1.34万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

I studied the intrafamilial transmission of mutans streptococci using DNA fingerprinting.1.Number of strain of mutans streptococci in oral cavity.The discrimination of homology of 700 strains of mutans streptococci separated from 35 persons in 9 families revealed that one to three species inhabit in oral cavity in many subjects. Approximately 48.6% of subjects have two species of mutans streptococci.2.The molecular biological proof concerning that mother is predominant mediator of transmission of mutans streptococci.Approximatelly 82.3% of children (14 children of 17) had the same strain found in their mothers. In these children three children had all of the same strains found in mothers. The strains of five children were transmitted from only their mothers and fathers. The homology of strains of seven children were not found in their parents. The strains of three children were the same of the strains of their fathers. These results show that strains of mutans streptococci in children were transmitted from their families. especially their mother at high probability.3.The biochemical specificity of mutans streptococci concerning of transmissionThe amount of synthesis of insoluble glucan in transmitted mutans streptococci was 1.10 to 2,47 more than untransmitted strains. The proposions of insoluble glucan contained in the synthesized glucan were higher in the transmitted strains at 79.1 to 90.5% than that of untransmitted strains indicated at 18.4 to 88.7%. The values of contact angle, shows hydrophobicity of bacteria, indicated smaller in all the transmitted strains below 14ﾟ than other strains. These findings revealed that the transmission of mutans streptococci should concern the adherent factors of insoluble glucan synthesis or contact angle.

我用DNA指纹技术研究了变异链球菌的家族内传播。口腔内变异链球菌株数。对9科35人分离的700株变形链球菌的同源性鉴定表明，许多受试者的口腔中存在1 ~ 3种变形链球菌。大约48.6%的受试者有两种变形链球菌。关于母亲是变异链球菌传播的主要媒介的分子生物学证据。大约82.3%的孩子（17个孩子中的14个）在他们的母亲身上发现了相同的菌株。在这些孩子中，有三个孩子的母亲身上发现了相同的菌株。5名儿童的病毒株仅由他们的父母传播。7例患儿在其父母中未发现菌株同源性。三个孩子的基因与他们父亲的基因相同。这些结果表明，儿童中的变形链球菌菌株是从其家庭传播的。尤其是他们的母亲。变异链球菌在传播方面的生化特异性：变异链球菌不溶性葡聚糖的合成量比未传播菌株多1.10 ~ 2.47。传染菌株合成的葡聚糖中不溶性葡聚糖的含量为79.1 ~ 90.5%，高于未传染菌株的18.4 ~ 88.7%。接触角值显示了细菌的疏水性，在14 / kg以下的所有传播菌株中，接触角值都小于其他菌株。这些结果表明，变形链球菌的传播可能与不溶性葡聚糖合成或接触角的粘附因素有关。