Elucidation of the settling of mutans streptococci to tooth-surface and the distribution using DNA fingerprinting.

本研究的目的是利用染色体DNA指纹（CDF）证明变异链球菌在日本家庭中的传播。对来自20个家庭（20对已婚夫妇和36名儿童）的76名受试者的牙菌斑培养的1940株分离菌进行CDF分析，鉴定出150株菌株。每个受试者有一个或多个至多4个菌株，每个家庭有两个或多个至多8个菌株。在74株患儿中，与母亲共见33株（44.6%），与父亲共见23株（31.1%），与父母双方共见2株（2.7%），与父母双方均未见（21.6%）。仅在两个家族中发现亲本间同源菌株。c型占67.3%，d型占12.7%，e型占12.0%，g型占8.0%。c型占48.5%，d、e、g型占87.8%。传染菌株的不溶性葡聚糖合成活性和疏水性均高于未传染菌株。这些结果表明，变形链球菌不仅可以从母亲那里获得，也可以从父亲和其他人那里获得。它还表明，细菌的特性也可能参与传播菌株。

The purpose of this study was to demonstrate the transmission of mutans streptococci in Japanese families using chromosomal DNA fingerprinting (CDF). The CDF analysis of 1,940 isolates cultured from the dental plaque of 76 subjects from 20 families (20 married couples and 36 children), resulted in identifying 150 strains. Each subject had one or more up to four strains and each family had two or more up to eight. For the 74 strains found in the children, 33 (44.6%) were common with their mothers, 23 (31.1%) were common with their fathers, 2 (2.7%) were common with both parents, and the remaining (21.6%) strains were not corresponded with both parents. Homological strains between parents were found in only two families. Of the differentiated strains 67.3% were serotype c, 12.7% were d, 12.0% were e, and 8.0% were g. Only 48.5% of serotype c, but 87.8% of serotype d, e, and g were transmitted strains. Transmitted strains showed higher activity levels for insoluble glucan synthesis and hydrophobicities than untransmitted strains. These results indicate that mutans streptococci can be derived not only from the mother but also from the father and other people. It also suggests that bacterial properties may also participate in transmitting strains.